Modification of poly(L-lactic acid)-based films and evaluation of physical and antibacterial properties by using multivariate data analysis

• Published in: International journal of biological macromolecules 2023-06, Vol.241, p.124583-124583, Article 124583
• Main Authors: Zahidova, Fidan, Yildiz, Semanur, Özdemir, Abdil, Gülfen, Mustafa, Yemiş, Gökçe Polat
• Format: Article
• Language: English
• Subjects: agar; Antibacterial activity; antibacterial properties; cluster analysis; Escherichia coli; Essential oil; essential oils; Green synthesis; methylene chloride; modulus of elasticity; multivariate analysis; nanosilver; permeability; PLA-based composite film; Poly(L-lactic acid); polyphenols; Silver nanoparticles; soybean oil; Spruce resin; Staphylococcus aureus; tensile strength; ultraviolet-visible spectroscopy; water vapor
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.124583

The aim of this study is to prepare new packaging materials with improved physical and antimicrobial properties that prevent the growth of microorganisms. Poly(L-lactic acid) (PLA) based packaging films were prepared by the solvent-casting method using spruce resin (SR), epoxidized soybean oil, an essential oil mixture (calendula and clove oil), and silver nanoparticles (AgNPs). The AgNPs were synthesized by the polyphenol reduction method, using spruce resin dissolved in methylene chloride. The prepared films were tested for antibacterial activity and physical properties, such as tensile strength (TS), elongation at break (EB), elastic modulus (EM), water vapor permeability (WVP), and UV-C blocking effect. The addition of SR decreased the water vapor permeation (WVP) of the films, whereas the addition of essential oils (EOs) increased this property due to their higher polarity. The morphological, thermal, and structural properties were characterized using SEM, UV–Visible spectroscopy, FTIR, and DSC. The agar disc well method showed that SR, AgNPs, and EOs imparted antibacterial activity to the PLA-based films against Staphylococcus aureus and Escherichia coli. Multivariate data analysis tools, such as principal component and hierarchical cluster analysis, were used to discriminate PLA-based films by simultaneously evaluating their physical and antibacterial properties. [Display omitted] •Poly(L-lactic acid) films were prepared by incorporating spruce resin, essential oils, and AgNPs.•The mechanical properties and antibacterial activity of the poly(L-lactic acid) films were examined.•Essential oils and AgNPs imparted antibacterial activity to poly(L-lactic acid)-based films.•The mixture of clove and calendula oils positively affected the physical properties of poly(L-lactic acid) films.•Multivariate data analysis tools were used to simultaneously evaluate the physical and antibacterial properties of films.