Untargeted Metabolomics Analyses and Contaminant Chemistry of Dreissenid Mussels at the Maumee River Area of Concern in the Great Lakes

• Published in: Environmental science & technology 2023-12, Vol.57 (48), p.19169-19179
• Main Authors: Legrand, Elena, Bayless, Amanda L, Bearden, Daniel W, Casu, Fabio, Edwards, Michael, Jacob, Annie, Johnson, W Edward, Schock, Tracey B
• Format: Article
• Language: English
• Subjects: Amino acids; Anthropogenic factors; anthropogenic stressors; Atrazine; biochemical pathways; Biomonitoring; Chemical contaminants; chemical pollutants; Chemical pollution; chemistry; Contaminants; Dreissena; Energy metabolism; Environmental health; environmental indicators; Environmental monitoring; Herbicides; Lakes; Liquid chromatography; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Metabolic response; Metabolism; metabolome; Metabolomics; Metolachlor; Mollusks; Mussels; NMR; NMR spectroscopy; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Organic contaminants; Pesticides; Polycyclic aromatic hydrocarbons; Rivers; Seasonal variations; Wastewater treatment; Wastewater treatment plants; Water analysis
• ISSN: 0013-936X; 1520-5851; 1520-5851
• DOI: 10.1021/acs.est.3c00812

Bivalves serve as an ideal ecological indicator; hence, their use by the NOAA Mussel Watch Program to monitor environmental health. This study aimed to expand the baseline knowledge of using metabolic end points in environmental monitoring by investigating the dreissenid mussel metabolome in the field. Dreissenids were caged at four locations along the Maumee River for 30 days. The mussel metabolome was measured using nuclear magnetic resonance spectroscopy, and mussel tissue chemical contaminants were analyzed using gas or liquid chromatography coupled with mass spectrometry. All Maumee River sites had a distinct mussel metabolome compared to the reference site and revealed changes in the energy metabolism and amino acids. Data also highlighted the importance of considering seasonality or handling effects on the metabolome at the time of sampling. The furthest upstream site presented a specific mussel tissue chemical signature of pesticides (atrazine and metolachlor), while a downstream site, located at Toledo's wastewater treatment plant, was characterized by polycyclic aromatic hydrocarbons and other organic contaminants. Further research into the dreissenid mussel's natural metabolic cycle and metabolic response to specific anthropogenic stressors is necessary before successful implementation of metabolomics in a biomonitoring program.