Utilizing outer membrane proteins (OMPs) as a vaccine to manage motile Aeromonas septicemia (MAS) in Nile tilapia (Oreochromis niloticus) with evaluating the vaccine immunoprotective effectiveness

• Published in: Aquaculture international 2024-12, Vol.32 (6), p.7235-7252
• Main Authors: Matter, Aya F., El Asley, Amel M., Abbass, Amany A., Shaheen, Adel A.
• Format: Article
• Language: English
• Subjects: Aeromonas; Aeromonas hydrophila; Bacteria; Biomedical and Life Sciences; Cytokines; Fish; Freshwater & Marine Ecology; Immunization; immunoglobulins; interleukins; Lesions; Life Sciences; Marine fishes; Molecular weight; Necrosis; neoplasms; Nitric oxide; Oreochromis niloticus; pathogenicity; Pathogens; polyacrylamide gel electrophoresis; Proteins; Sepsis; Septicaemia; septicemia; Tilapia; Vaccines; Whitefish; Zoology
• ISSN: 0967-6120; 1573-143X
• DOI: 10.1007/s10499-024-01511-6

Motile aeromonads are an opportunistic and heterogenic bacteria that cause motile Aeromonas septicemia (MAS) which is accompanied with variety of clinical signs and lesions in fish. It has been found that their outer membrane proteins (OMPs) are highly immunogenic and may be used as a vaccine candidate. The present study was undertaken to evaluate OMP protection against Aeromonas hydrophila ( A. hydrophila ) strain ATCC 7966 ( A 5 and A 8) and A. hydrophila strain DSM 30,187 ( A 6). Pathogenicity tests revealed that out of 12 isolated strains, seven were highly pathogenic, three were moderate, and two were avirulent. SDS-PAGE analysis of OMPs showed several protein bands (7–22) of different molecular weights ranging from 14 to 346 kDa. The common protein band was 39 kDa. It seems that pathogenicity and the number of protein bands are related in the highly pathogenic strains. Challenged A. hydrophila for Nile tilapia ( O. niloticus ) previously actively immunized with OMPs ( A 4, A 5, A 6, A8 , and A 11) showed a significant increase in relative percent survival (RPS) ranging from 50 to 100%. Immunological parameters such as immunoglobulin M (IgM), antioxidants (SOD, GSH, and CAT), and nitric oxide (NO) and expression of pro-inflammatory cytokines, interleukin 1β (IL-1β), and tumor necrosis factor-α (TNFα) showed a significant increase in OMP-immunized fish. Challenged A. hydrophila for O. niloticus previously passively immunized with OMPs ( A 4, A 5, A 6, A8, and A11 ) in fish-raised sera showed a significant increase in RPS (20 to 100%) in the vaccinated groups. It is concluded that OMPs from Aeromonas spp. have been identified as suitable target for protection development, especially in the case of heterogenic bacteria.