Bioactive phytochemicals from the tubers of Bletilla striata Rchb.f

• Published in: Natural product research 2024-11, Vol.38 (21), p.3848-3853
• Main Authors: Lee, Chia-Lin, Jhan, Yun-Lian, Chiang, Hsiu-Mei, Chen, Chao-Jung
• Format: Article
• Language: English
• Subjects: Animals; anti-melanogenesis; anti-ROS generation; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Bibenzyls - chemistry; Bibenzyls - pharmacology; Biocompatibility; Bletilla striata; Bletilla striata Rchb.f; Cell Line, Tumor; cinnamates; Cytotoxicity; Dihydrophenanthrene; fatty acids; HaCaT Cells; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Hyperpigmentation; Inhibitory Concentration 50; Melanins; melanogenesis; Melanoma; Melanoma, Experimental - drug therapy; Mice; Molecular Structure; Orchidaceae; Orchidaceae - chemistry; phenanthrenes; Phenanthrenes - chemistry; Phenanthrenes - isolation & purification; Phenanthrenes - pharmacology; phytochemical analysis; Phytochemicals; Phytochemicals - chemistry; Phytochemicals - isolation & purification; Phytochemicals - pharmacology; Plant Tubers - chemistry; Reactive Oxygen Species - metabolism; Skin diseases; Tubers
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2023.2261605

Thirty-four phytochemicals were isolated from dry tubers of Bletilla striata Rchb.f. The compounds were classified as bibenzyls 1-14, dihydrophenanthrenes 15, 17, 20, 21, phenanthrenes 16, 18, 19, simple benzoids 22-24, a fatty acid 25, glucosyloxybenzyl 2-isobutylmalates 26-32, and glucosyloxybenzyl cinnamates 33, 34. Compounds 1-4, 7, 8, 11, 12, and 16 inhibited melanogenesis (17.96-55.27%) induced by α-MSH in B16F10 cells at 10-40 μM. However, compounds 9, 10, 17, 18, and 21 exhibited significant cytotoxicity against melanomas, with IC 50 values of 12-34 μM. Additionally, compounds 15, 17, 19, 20, 23, 31, and 33 reduced the ROS generation induced by H 2 O 2 in HaCaT cells at 6.25-100 μM. In particular, compounds 15, 19, and 20 strongly inhibited ROS generation, with IC 50 values of 2.15-9.48 μM. Consequently, compounds 1-4, 7-12, and 15-21 may be the strongest leads to follow in B. striata extract for further research on the skin disorders, hyperpigmentation, melanoma, and ageing.