Helicobacter cappadocius sp. nov., from lizards: The first psychrotrophic Helicobacter species

• Published in: Systematic and applied microbiology 2024-11, Vol.47 (6), p.126557, Article 126557
• Main Authors: Aydin, Fuat, Tarhane, Serdal, Karakaya, Emre, Abay, Seçil, Kayman, Tuba, Güran, Özgür, Bozkurt, Emin, Üzüm, Nazan, Avci, Aziz, Olgun, Kurtuluş, Jablonski, Daniel, Güran, Cansu, Burçin Saticioğlu, İzzet
• Format: Article
• Language: English
• Subjects: amphibians; Animals; Bacterial Typing Techniques; Base Composition; cloaca; Cloaca - microbiology; DNA, Bacterial - genetics; genes; Genome, Bacterial - genetics; genus; Helicobacter; Helicobacter - classification; Helicobacter - genetics; Helicobacter - isolation & purification; Helicobacter Infections - microbiology; Helicobacter Infections - veterinary; Helicobacter mustelae; host specificity; Lizard; lizards; Lizards - microbiology; metagenomics; microbiology; Novel species; nucleotide sequences; Overall genome-related index; phenotype; Phylogeny; Psychrotrophic; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; species; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0723-2020; 1618-0984; 1618-0984
• DOI: 10.1016/j.syapm.2024.126557

It was aimed to determine the prevalence of Helicobacter in some reptilian and amphibian species in Türkiye and to describe the bacteria. For this purpose, 73 cloacal swab samples were used as material. The description of the isolates was performed by detailed phenotypic tests, whole genome analyses, and MALDI-TOF MS. As a result of the phenotypic analysis, two helical, curved Gram-negative, motile isolates were recovered. It was determined through the analysis of 16S rRNA gene sequences that two isolates belonged to the genus Helicobacter. These isolates were found to be in a distinct group from other Helicobacter species. However, the 16S rRNA sequence did not match any identified species, with the closest match being Helicobacter mustelae strain R85-13-6T, which had an identity level of 96.2 %. Additionally, it was found that strains faydin-H75T and faydin-H76 had a 99.3 % identity level for their 16S rRNA genes. After conducting dDDH and ANI analyses, it was found that strains faydin-H75T and their close neighbors H.anseris ATCC BAA-1299T shared 13.5 % and 68.8 % similarity, respectively. The genome size of the strains was 1.7 Mb while G + C contents were 33.5 %. Metagenomic analyses using IMNGS and Protologger tools revealed the presence of faydin-H75T in various lizard species with high similarity, confirming its broad distribution and host specificity. The results indicated that these two strains represent a novel species, for which we propose the name Helicobacter cappadocius with faydin-H75T (=NCTC014972 = LMG 33382 = DSM117062) as the respective type strain. The current novel species is the first Helicobacter species to exhibit a psychrotrophic feature.