Positive effect of peptide‐calcium chelates from Grifola frondosa on a mouse model of senile osteoporosis

Calcium supplementation has been shown to be efficacious in mitigating the progression of senile osteoporosis (SOP) and reducing the incidence of osteoporotic fractures resulting from prolonged calcium shortage. In this study, Grifola frondosa (GF) peptides‐calcium chelate were synthesized through t...

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Published in:Journal of food science 2024-06, Vol.89 (6), p.3816-3828
Main Authors: Xiong, Yu, Li, Jing‐Ru, Peng, Pei‐Zhi, Liu, Bin, Zhao, Li‐Na
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Animals
biochemical pathways
blood serum
bones
calcium
Calcium - metabolism
Calcium Chelating Agents - pharmacology
Calcium chloride
Chelates
Chelation
Disease Models, Animal
Electron microscopes
Extracellular matrix
Female
femoral proteome
focal adhesions
food science
Fourier transforms
Fractures
Galactose
gene expression regulation
Grifola frondosa
Humans
Infrared spectrophotometers
Metabolic pathways
Mice
Molecular weight
Osteoporosis
Peptides
Peptides - pharmacology
peptide‐calcium chelates
Scanning electron microscopy
senile osteoporosis
Signal transduction
Spectrophotometry
spectroscopy
Western blotting
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Summary:Calcium supplementation has been shown to be efficacious in mitigating the progression of senile osteoporosis (SOP) and reducing the incidence of osteoporotic fractures resulting from prolonged calcium shortage. In this study, Grifola frondosa (GF) peptides‐calcium chelate were synthesized through the interaction between peptide from GF and CaCl2. The chelation reaction was shown to involve the participation of the amino and carboxyl groups in the peptide, as revealed by scanning electron microscope, Fourier‐transform infrared, and ultraviolet spectrophotometry. Furthermore, a mouse model of (SOP) induced by d‐galactose was established (SCXK‐2018‐0004). Results demonstrated that low dosage of low‐molecular weight GF peptides‐calcium chelates (LLgps‐Ca) could significantly improve serum index and pathological features of bone tissue and reduce bone injury. Further research suggested that LLgps‐Ca could ameliorate SOP by modulating the disrupted metabolic pathway, which includes focal adhesion, extracellular matrix receptor interaction, and PI3K‐Akt signaling pathway. Using Western blot, the differentially expressed proteins were further confirmed. Thus, calciumchelating peptides from GF could serve as functional calcium agents to alleviate SOP.
ISSN:0022-1147
1750-3841
1750-3841
DOI:10.1111/1750-3841.17073