Positive effect of peptide‐calcium chelates from Grifola frondosa on a mouse model of senile osteoporosis

• Published in: Journal of food science 2024-06, Vol.89 (6), p.3816-3828
• Main Authors: Xiong, Yu, Li, Jing‐Ru, Peng, Pei‐Zhi, Liu, Bin, Zhao, Li‐Na
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; biochemical pathways; blood serum; bones; calcium; Calcium - metabolism; Calcium Chelating Agents - pharmacology; Calcium chloride; Chelates; Chelation; Disease Models, Animal; Electron microscopes; Extracellular matrix; Female; femoral proteome; focal adhesions; food science; Fourier transforms; Fractures; Galactose; gene expression regulation; Grifola frondosa; Humans; Infrared spectrophotometers; Metabolic pathways; Mice; Molecular weight; Osteoporosis; Peptides; Peptides - pharmacology; peptide‐calcium chelates; Scanning electron microscopy; senile osteoporosis; Signal transduction; Spectrophotometry; spectroscopy; Western blotting
• ISSN: 0022-1147; 1750-3841; 1750-3841
• DOI: 10.1111/1750-3841.17073

Calcium supplementation has been shown to be efficacious in mitigating the progression of senile osteoporosis (SOP) and reducing the incidence of osteoporotic fractures resulting from prolonged calcium shortage. In this study, Grifola frondosa (GF) peptides‐calcium chelate were synthesized through the interaction between peptide from GF and CaCl2. The chelation reaction was shown to involve the participation of the amino and carboxyl groups in the peptide, as revealed by scanning electron microscope, Fourier‐transform infrared, and ultraviolet spectrophotometry. Furthermore, a mouse model of (SOP) induced by d‐galactose was established (SCXK‐2018‐0004). Results demonstrated that low dosage of low‐molecular weight GF peptides‐calcium chelates (LLgps‐Ca) could significantly improve serum index and pathological features of bone tissue and reduce bone injury. Further research suggested that LLgps‐Ca could ameliorate SOP by modulating the disrupted metabolic pathway, which includes focal adhesion, extracellular matrix receptor interaction, and PI3K‐Akt signaling pathway. Using Western blot, the differentially expressed proteins were further confirmed. Thus, calciumchelating peptides from GF could serve as functional calcium agents to alleviate SOP.