Screening of novel disease genes of sepsis-induced myocardial Disfunction by RNA sequencing and bioinformatics analysis

• Published in: Genomics (San Diego, Calif.) Calif.), 2024-09, Vol.116 (5), p.110911, Article 110911
• Main Authors: Yao, Hanyi, Xiao, Zixi, Liu, Shufang, Gao, Xingjian, Wu, Zehong, Li, Dongping, Yi, Zhangqing, Zhou, Haojie, Zhang, Weizhi
• Format: Article
• Language: English
• Subjects: Animals; Bioinformatic analysis; bioinformatics; Bucladesine; Cardiomyopathies - genetics; Cardiomyopathies - metabolism; Computational Biology - methods; Cross-species; data collection; drugs; gene expression regulation; genomics; Humans; hydrogen; Molecular Docking Simulation; Novel disease genes; pathogenesis; Protein Glutamine gamma Glutamyltransferase 2 - metabolism; RNA; RNA sequencing; Sepsis - genetics; Sepsis-induced myocardial dysfunction; therapeutics; Tissue Inhibitor of Metalloproteinase-1 - genetics; Tissue Inhibitor of Metalloproteinase-1 - metabolism; Transglutaminases - chemistry; Transglutaminases - genetics; Transglutaminases - metabolism
• ISSN: 0888-7543; 1089-8646; 1089-8646
• DOI: 10.1016/j.ygeno.2024.110911

There is still a lack of effective treatment for sepsis-induced myocardial dysfunction (SIMD), while the pathogenesis of SIMD still remains largely unexplained. RNA sequencing results (GSE267388 and GSE79962) were used for cross-species integrative analysis. Bioinformatic analyses were used to delve into function, tissue- and cell- specificity, and interactions of genes. External datasets and qRT-PCR experiments were used for validation. L1000 FWD was used to predict targeted drugs, and 3D structure files were used for molecular docking. Based on bioinformatic analyses, ten differentially expressed genes were selected as genes of interest, seven of which were verified to be significantly differential expression. Bucladesine was considered as a potential targeted drug for SIMD, which banded to seven target proteins primarily by forming hydrogen bonds. It was considered that Cebpd, Timp1, Pnp, Osmr, Tgm2, Cp, and Asb2 were novel disease genes, while bucladesine was a potential therapeutic drug, of SIMD. •The pathogenesis of sepsis-induced myocardial dysfunction remains largely unexplained.•This study produced a new RNA sequencing dataset of heart tissue from mouse models.•Seven novel disease genes of sepsis-induced myocardial dysfunction were screened.•Bucladesine was considered as a potential targeted drug of these genes.