Efficient and transformation‐free genome editing in pepper enabled by RNA virus‐mediated delivery of CRISPR/Cas9

Tomato spotted wilt virus‐mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA‐free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties an...

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Bibliographic Details
Published in:Journal of integrative plant biology 2024-10, Vol.66 (10), p.2079-2082
Main Authors: Zhao, Chenglu, Lou, Huanhuan, Liu, Qian, Pei, Siqi, Liao, Qiansheng, Li, Zhenghe
Format: Article
Language:English
Subjects:
Capsicum - genetics
Capsicum - virology
Capsicum annuum
CRISPR
CRISPR-Cas systems
CRISPR-Cas Systems - genetics
CRISPR/Cas9
Editing
Gene Editing - methods
Genetic transformation
genome
genome editing
Genome, Plant - genetics
pepper
plant biology
Plant viruses
regeneration
RNA
RNA editing
RNA viruses
RNA Viruses - genetics
Tissue culture
tomato spotted wilt virus
Tomatoes
Tospovirus - genetics
Tospovirus - physiology
transient delivery
Vegetables
viral vector
Wilt
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Summary:Tomato spotted wilt virus‐mediated delivery of CRISPR/Cas9 bypasses the need for stable transformation and permits efficient, DNA‐free genome editing in pepper. Remarkably, up to 77.9% of regenerated pepper plants contained heritable edits. This method has been validated with two pepper varieties and is compatible with existing tissue culture protocols.
ISSN:1672-9072
1744-7909
1744-7909
DOI:10.1111/jipb.13741