Isolation, characterization, and application of a novel Pseudomonas fluorescens phage vB_PF_Y1-MI in contaminated milk

• Published in: Molecular genetics and genomics : MGG 2024-12, Vol.299 (1), p.97-97, Article 97
• Main Authors: Xuan, Guanhua, Liu, Xianjun, Wang, Yinfeng, Lin, Hong, Jiang, Xiuping, Wang, Jingxue
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Animals; bacteriophages; Bacteriophages - classification; Bacteriophages - genetics; Bacteriophages - isolation & purification; Base Composition - genetics; Biochemistry; Biological control; biological control agents; Biomedical and Life Sciences; Burst size; Caudoviricetes; Cell size; class; DNA; face; Food contamination; Food contamination & poisoning; Food Contamination - prevention & control; Food industry; Food Microbiology; genome; Genome, Viral - genetics; genomics; Human Genetics; Latent period; Life Sciences; Lysogeny; Microbial Genetics and Genomics; Milk - microbiology; Milk - virology; milk contamination; Open Reading Frames - genetics; Original Article; Phages; Phylogeny; Plant Genetics and Genomics; Pseudomonas fluorescens; Pseudomonas fluorescens - genetics; Pseudomonas fluorescens - virology; Pseudomonas Phages - genetics; Pseudomonas Phages - isolation & purification; temperature; tRNA; Virulence
• ISSN: 1617-4615; 1617-4623; 1617-4623
• DOI: 10.1007/s00438-024-02179-6

The food industry has incurred substantial losses from contamination by Pseudomonas fluorescens , emphasizing the critical importance of implementing effective control strategies. Phages are potential sterilizers due to their specific killing abilities and the difficulty bacteria face in developing resistance. However, a significant barrier to their development is the lack of diversity among phage types. In this study, we characterized a novel lytic P. fluorescens phage, named vB_PF_Y1-MI. Phage vB_PF_Y1-MI displayed a latent period of nearly 10 min and a high burst size of 1493 PFU/cell. This phage showed good activity over a wide range of temperature (up to 70 °C) and pH (3–12). The genome of phage vB_PF_Y1-MI spans 93,233 bp with a GC content of 45%. It encompasses 174 open-reading frames and 19 tRNA genes, while no lysogeny or virulence-associated genes were detected. Phylogenetic analysis positions it as a novel unassigned evolutionary lineage within the Caudoviricetes class among related dsDNA phages. Our study provides foundational insights into vB_PF_Y1-MI and emphasizes its potential as an effective biological control agent against P. fluorescens . This research offers crucial theoretical groundwork and technical support for subsequent efforts in preventing and controlling P. fluorescens contamination.