Green synthesized extracts/Au complex of Phyllospongia lamellosa: Unrevealing the anti-cancer and anti-bacterial potentialities, supported by metabolomics and molecular modeling

• Published in: Heliyon 2024-07, Vol.10 (13), p.e34000, Article e34000
• Main Authors: Aati, Sultan, Aati, Hanan Y., El-Shamy, Sherine, Khanfar, Mohammad A., A.Naeim, Mohamed A.Ghani, Hamed, Ahmed A., Rateb, Mostafa E., Hassan, Hossam M., Aboseada, Mahmoud A.
• Format: Article
• Language: English
• Subjects: amides; Anti-Bacterial; Anti-Cancer; antibacterial properties; breast neoplasms; bromination; cytotoxicity; Escherichia coli; ethyl acetate; Fourier transform infrared spectroscopy; gold; Gold nanoparticles; lipophilicity; Metabolomics; Molecular docking; molecular dynamics; nanogold; neoplasm cells; Phyllospongia lamellosa; Red Sea; secondary metabolites; terpenoids; transmission electron microscopy; ultraviolet-visible spectroscopy
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e34000

The anti-cancer and anti-bacterial potential of the Red Sea sponge Phyllospongia lamellosa in its bulk (crude extracts) and gold nanostructure (loaded on gold nanaoparticles) were investigated. Metabolomics analysis was conducted, and subsequently, molecular modeling studies were conducted to explore and anticipate the P. lamellosa secondary metabolites and their potential target for their various bioactivities. The chloroformic extract (CE) and ethyl acetate extract (EE) of the P. lamellosa predicted to include bioactive lipophilic and moderately polar metabolites, respectively, were used to synthesize gold nanoparticles (AuNPs). The prepared AuNPs were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and UV–vis spectrophotometric analyses. The cytotoxic activities were tested against MCF-7, MDB-231, and MCF-10A. Moreover, the anti-bacterial, antifungal, and anti-biofilm activity were assessed. Definite classes of metabolites were identified in CE (terpenoids) and EE (brominated phenyl ethers and sulfated fatty amides). Molecular modeling involving docking and molecular dynamics identified Protein-tyrosine phosphatase 1B (PTP1B) as a potential target for the anti-cancer activities of terpenoids. Moreover, CE exhibited the most powerful activity against breast cancer cell lines, matching our molecular modeling study. On the other hand, only EE was demonstrated to possess powerful anti-bacterial and anti-biofilm activity against Escherichia coli. In conclusion, depending on their bioactive metabolites, P. lamellosa-derived extracts, after being loaded on AuNPs, could be considered anti-cancer, anti-bacterial, and anti-biofilm bioactive products. Future work should be completed to produce drug leads.