Association of Neuroblastoma (NB) SH-SY5Y Cells with Antibodies of Parasitic Origin (Anti- Acanthamoeba and Anti- Toxocara canis )

It is little known that trophozoites and eggs can reduce tumors in vitro and animal models. Although this has been known for many years, the mechanism that induces the antitumor effect in these parasites is still not known. We employed Western blot (WB) and immunofluorescence (IFC) by confocal micro...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-12, Vol.25 (24), p.13577
Main Authors: Maravelez Acosta, Víctor Alberto, Garcia, Maria de Lourdes Caballero, Patiño López, Genaro, Crisóstomo Vázquez, María Del Pilar, Franco Sandoval, Luz Ofelia, Eligio García, Leticia
Format: Article
Language:English
Subjects:
Acanthamoeba - immunology
Acanthamoeba - metabolism
Animals
Antibodies
Antibodies, Helminth - immunology
Antibodies, Protozoan - immunology
Antigens
Apoptosis
Breast cancer
Cancer therapies
Cell Line, Tumor
Cytotoxicity
Humans
Immunotherapy
Infections
Medical research
Melanoma
Metastasis
Microorganisms
Morphology
Neuroblastoma
Neuroblastoma - immunology
Neuroblastoma - metabolism
Neuroblastoma - pathology
Parasites
Peptides
Prostate cancer
Proteins
Protozoa
Toxocara canis - immunology
Toxocara canis - metabolism
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Summary:It is little known that trophozoites and eggs can reduce tumors in vitro and animal models. Although this has been known for many years, the mechanism that induces the antitumor effect in these parasites is still not known. We employed Western blot (WB) and immunofluorescence (IFC) by confocal microscopy to explore the potential protein binding between neuroblastoma (NB) SH-SY5Y cells and anti- and anti- antibodies. Using WB, we detected two fragments of 70 kDa and 60 kDa recognized by the anti- antibodies, and two fragments of 115 kDa and 70 kDa recognized by the anti- antibodies. In both cases, the IFC results were positive in the cell membrane of the SH-SY5Y cells. Our findings suggest a potential overlap of similar molecules between these parasites and tumor cells, which may contribute to tumor elimination. Investigating the relationship between anti- and anti- antibodies in neoplastic cells could provide evidence for the future use of these anti-parasitic antibodies in targeting NB or other cancers.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252413577