Proteomic analysis of the human amniotic mesenchymal stromal cell secretome by integrated approaches via filter-aided sample preparation

The immunomodulatory, anti-inflammatory and regenerative properties of the human amniotic mesenchymal stromal cells (hAMSCs) secretome are acknowledged but the understanding of the specific bioactive components remains incomplete. To address these limitations, the present investigation aimed to prof...

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Bibliographic Details
Published in:Journal of proteomics 2025-01, Vol.310, p.105339, Article 105339
Main Authors: Muntiu, Alexandra, Papait, Andrea, Vincenzoni, Federica, Rossetti, Diana Valeria, Romele, Pietro, Cargnoni, Anna, Silini, Antonietta, Parolini, Ornella, Desiderio, Claudia
Format: Article
Language:English
Subjects:
collagen
digestion
Filter-aided sample preparation
fractionation
glyceraldehyde-3-phosphate dehydrogenase
Human amniotic mesenchymal stromal cells
humans
medicine
mesenchymal stromal cells
molecular weight
peptides
Peptidomics
phosphopyruvate hydratase
proteome
Proteomics
Secretome
therapeutics
thymosin
tissue repair
trypsin
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Summary:The immunomodulatory, anti-inflammatory and regenerative properties of the human amniotic mesenchymal stromal cells (hAMSCs) secretome are acknowledged but the understanding of the specific bioactive components remains incomplete. To address these limitations, the present investigation aimed to profile the proteins and peptides content of the hAMSC secretome through sample pretreatment and fractionation on 10 kDa molecular cut-off FASP (Filter Aided Sample Preparation) device and LC-MS analysis. The filter retained protein fraction underwent trypsin digestion, while the unretained was collected unchanged for intact small proteins and peptides analysis. This combined approach (C-FASP) collects in a single step two complementary fractions, advantageously saving sample volume and time of analysis. The bottom-up analysis of the C-FASP proteins fraction >10 kDa confirmed our previous findings, establishing a set of proteins consistently characterizing the hAMSC secretome. The analysis of the fraction
ISSN:1874-3919
DOI:10.1016/j.jprot.2024.105339