Candidate antibody reference reagents for Chlamydia trachomatis serology

• Published in: Journal of immunological methods 2024-11, Vol.534, p.113761, Article 113761
• Main Authors: da Silva, Filomeno Coelho, Kamuyu, Gathoni, Michels, Birgitta, Edney, Jessica, Hassall, Laura, Stickings, Paul, Maharjan, Sunil, Waterboer, Tim, Beddows, Simon
• Format: Article
• Language: English
• Subjects: Adult; antibodies; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antibody; antigens; Antigens, Bacterial - immunology; Bacterial Outer Membrane Proteins - immunology; Bacterial Proteins - immunology; burden of disease; Chlamydia Infections - diagnosis; Chlamydia Infections - immunology; Chlamydia Infections - microbiology; Chlamydia trachomatis; Chlamydia trachomatis - immunology; Female; Humans; MOMP; outer membrane proteins; pgp3; Porins - immunology; quality control; Reference reagent; Reference Standards; Serologic Tests - methods; Serologic Tests - standards; Serology; virulence; Young Adult
• ISSN: 0022-1759; 1872-7905; 1872-7905
• DOI: 10.1016/j.jim.2024.113761

Chlamydia trachomatis (Ct) serology is an important tool for monitoring infection and disease burden but there are currently no formal reference reagents to harmonize results reporting. Our objective was to develop a panel of candidate reference reagents with reactivity against the major outer membrane protein (MOMP) and virulence factor (pgp3) antigens. Plasma packs from females (20–40 years old) were screened against MOMP and pgp3 antigens and selected positive and negative samples pooled to create a panel of candidate antibody reference reagents that were tested in two laboratories. Antigen specificity and internal quality assurance were also evaluated. Suitable candidate materials have been selected to produce Ct reference reagents.