Therapeutic potential of platelet rich plasma against experimental Cryptosporidium parvum infection: in vivo study in immunosuppressed mice

• Published in: Journal of parasitic diseases 2024-12, Vol.48 (4), p.849-859
• Main Authors: Mahmoud, Nada R., Younis, Azza I., Zalat, Rabab S., Soliman, Ahmed S. A., Khater, Mona M.
• Format: Article
• Language: English
• Subjects: albino; Cryptosporidiosis; Cryptosporidium parvum; Health Promotion and Disease Prevention; histopathology; immunohistochemistry; Infections; Infectious Diseases; intestines; intravenous injection; Lamina propria; males; Medicine; Medicine & Public Health; oocysts; Original Article; Platelets; research institutions; Small intestine; Transforming growth factor-b1
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-024-01713-y

The present study was designed to assess the possible effects of platelet rich plasma (PRP) when used individually and in combination with nitazoxanide (NTZ) on experimental Cryptosporidium parvum ( C. parvum ) infection. It was conducted on 100 male albino mice, laboratory bred in Theodore Bilharz Research Institute. Starting from the 7th day post infection (p.i), therapeutics were given to immunosuppressed infected mice, which were divided as follows; oral NTZ treated group (0.2 mg/g/day for 6 consecutive days), six-PRP-treated groups (0.5 μl/g/week) to be administered intravenously (IV) in 1st, 2nd, 3rd week as PRP alone in (3 groups) and combined with oral NTZ (0.2 mg/g/day for 6 consecutive days) in (3 groups). Parasitological, histopathological and immunohistochemical assessments of therapeutics under study were done. Fecal pellets collected from groups at different intervals were stained using modified Ziehl–Neelsen and examined under microscope. Among PRP-treated groups, the highest significant percentage of oocyst reduction (89.96%) was observed in the group received 3 doses of PRP in combination with NTZ on the 35th day post infection. Likewise, the histopathological examination of small intestinal tissue sections showed improvement in villous architecture with mild to moderate stunting and moderate inflammatory infiltrates in lamina propria. Immunohistochemical staining of small intestinal tissue sections showed moderate increase in the expression of TGF-β1. Therefore, PRP can be a novel strategy in the treatment of cryptosporidiosis particularly when combined with NTZ.