New insights into Microsporidia polar tube function and invasion mechanism

• Published in: The Journal of eukaryotic microbiology 2024-09, Vol.71 (5), p.e13043-n/a
• Main Authors: Fayet, Maurine, Long, Mengxian, Han, Bing, Belkorchia, Abdel, Delbac, Frédéric, Polonais, Valerie
• Format: Article
• Language: English
• Subjects: Animals; Cytoplasmic organelles; energy; extrusion; Fungal Proteins - genetics; Fungal Proteins - metabolism; genome; Genomes; host cell invasion; Host-Pathogen Interactions; Humans; invasion mechanisms; invertebrates; microbiology; Microsporidia; Microsporidia - classification; Microsporidia - genetics; Microsporidia - physiology; Organelles; Parasite resistance; Parasites; polar tube structure; Protein transport; Spores; Spores, Fungal - physiology; sporoplasm transfer; Tomography; vertebrates
• ISSN: 1066-5234; 1550-7408; 1550-7408
• DOI: 10.1111/jeu.13043

Microsporidia comprise a large phylum of single‐cell and obligate intracellular parasites that can infect a wide range of invertebrate and vertebrate hosts including humans. These fungal‐related parasites are characterized by a highly reduced genome, a strong energy dependence on their host, but also by their unique invasion organelle known as the polar tube which is coiled within the resistant spore. Upon appropriate environmental stimulation, the long hollow polar tube (ranging from 50 to 500 μm in length) is extruded at ultra‐fast speeds (300 μm/s) from the spore acting as a harpoon‐like organelle to transport and deliver the infectious material or sporoplasm into the host cell. To date, seven polar tube proteins (PTPs) with distinct localizations along the extruded polar tube have been described. For example, the specific location of PTP4 and PTP7 at the tip of the polar tube supports their role in interacting with cellular receptor(s). This chapter provides a brief overview on the current understanding of polar tube structure and dynamics of extrusion, primarily through recent advancements in cryo‐tomography and 3D reconstruction. It also explores the various mechanisms used for host cell invasion. Finally, recent studies on the structure and maturation of sporoplasm and its moving through the tube are discussed.