The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot

The most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybea...

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Published in:Plant pathology 2024-12, Vol.73 (9), p.2429-2439
Main Authors: Liu, Haixu, Zhang, Zhuoqun, Xu, Ying, Du, Xiuming, Wen, Jingzhi
Format: Article
Language:English
Subjects:
Ammonia
Bacillomycin
Bacillus amyloliquefaciens
Biological control
biological control agents
Biological effects
catechol oxidase
Cellulase
Chitinase
Disease control
Encystment
endo-1,4-beta-glucanase
Fermentation
Filtrate
filtrates
Genes
Germination
Mycelia
mycelium
oospores
Pathogenesis
Peroxidase
Phenylalanine
phenylalanine ammonia-lyase
Phytophthora
Phytophthora root rot
Phytophthora sojae
plant height
plant pathology
Polyphenol oxidase
proteinases
Rhizosphere
Root rot
siderophores
soil
Soil microorganisms
Soil resistance
Soil-borne diseases
soybean
Soybeans
Surfactin
zoospores
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Zhang, Zhuoqun
Xu, Ying
Du, Xiuming
Wen, Jingzhi
description The most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p 
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Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p &lt; 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p &lt; 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p &lt; 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives. A Bacillus amyloliquefaciens strain, W82T‐44, was isolated from the rhizosphere soil of a resistant soybean variety. It exhibits the dual effects of promoting soybean growth and preventing Phytophthora root rot.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1111/ppa.13994</identifier><language>eng</language><publisher>Oxford: Wiley Subscription Services, Inc</publisher><subject>Ammonia ; Bacillomycin ; Bacillus amyloliquefaciens ; Biological control ; biological control agents ; Biological effects ; catechol oxidase ; Cellulase ; Chitinase ; Disease control ; Encystment ; endo-1,4-beta-glucanase ; Fermentation ; Filtrate ; filtrates ; Genes ; Germination ; Mycelia ; mycelium ; oospores ; Pathogenesis ; Peroxidase ; Phenylalanine ; phenylalanine ammonia-lyase ; Phytophthora ; Phytophthora root rot ; Phytophthora sojae ; plant height ; plant pathology ; Polyphenol oxidase ; proteinases ; Rhizosphere ; Root rot ; siderophores ; soil ; Soil microorganisms ; Soil resistance ; Soil-borne diseases ; soybean ; Soybeans ; Surfactin ; zoospores</subject><ispartof>Plant pathology, 2024-12, Vol.73 (9), p.2429-2439</ispartof><rights>2024 British Society for Plant Pathology.</rights><rights>Plant Pathology © 2024 British Society for Plant Pathology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2204-260d3e295da3cadc9e859169407a3be7febafef81e9e80f41a2a102a0511ee7f3</cites><orcidid>0000-0002-5521-7034</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Liu, Haixu</creatorcontrib><creatorcontrib>Zhang, Zhuoqun</creatorcontrib><creatorcontrib>Xu, Ying</creatorcontrib><creatorcontrib>Du, Xiuming</creatorcontrib><creatorcontrib>Wen, Jingzhi</creatorcontrib><title>The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot</title><title>Plant pathology</title><description>The most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p &lt; 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p &lt; 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p &lt; 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives. A Bacillus amyloliquefaciens strain, W82T‐44, was isolated from the rhizosphere soil of a resistant soybean variety. 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Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p &lt; 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p &lt; 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p &lt; 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives. A Bacillus amyloliquefaciens strain, W82T‐44, was isolated from the rhizosphere soil of a resistant soybean variety. It exhibits the dual effects of promoting soybean growth and preventing Phytophthora root rot.</abstract><cop>Oxford</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/ppa.13994</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-5521-7034</orcidid></addata></record>
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ispartof Plant pathology, 2024-12, Vol.73 (9), p.2429-2439
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source Wiley
subjects Ammonia
Bacillomycin
Bacillus amyloliquefaciens
Biological control
biological control agents
Biological effects
catechol oxidase
Cellulase
Chitinase
Disease control
Encystment
endo-1,4-beta-glucanase
Fermentation
Filtrate
filtrates
Genes
Germination
Mycelia
mycelium
oospores
Pathogenesis
Peroxidase
Phenylalanine
phenylalanine ammonia-lyase
Phytophthora
Phytophthora root rot
Phytophthora sojae
plant height
plant pathology
Polyphenol oxidase
proteinases
Rhizosphere
Root rot
siderophores
soil
Soil microorganisms
Soil resistance
Soil-borne diseases
soybean
Soybeans
Surfactin
zoospores
title The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot
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