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Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches

Mandevilla velutina (Mart. Ex Stadelm.) Woodson, known in Brazil as "infalível" and "jalapa", is a medicinal plant native from the Cerrado region (Brazilian Savannah). The underground organ (xylopodium) of this species is prepared as ethanolic extract or infusion and it is common...

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Published in:Journal of ethnopharmacology 2025-01, Vol.337 (Pt 2), p.118994, Article 118994
Main Authors: Silva, Regildo Márcio Gonçalves da, Barbosa, Fernando Cesar, Santos, Hugo Henrique, Granero, Filipe Oliveira, Figueiredo, Célia Cristina Malaguti, Nicolau-Junior, Nilson, Hamaguchi, Amélia, Silva, Luciana Pereira
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container_title Journal of ethnopharmacology
container_volume 337
creator Silva, Regildo Márcio Gonçalves da
Barbosa, Fernando Cesar
Santos, Hugo Henrique
Granero, Filipe Oliveira
Figueiredo, Célia Cristina Malaguti
Nicolau-Junior, Nilson
Hamaguchi, Amélia
Silva, Luciana Pereira
description Mandevilla velutina (Mart. Ex Stadelm.) Woodson, known in Brazil as "infalível" and "jalapa", is a medicinal plant native from the Cerrado region (Brazilian Savannah). The underground organ (xylopodium) of this species is prepared as ethanolic extract or infusion and it is commonly used in traditional medicine to treat snake venom. Although, locals and indigenous populations from Cerrado have used M. velutina for the treatment of infection by Trypanosoma cruzi (Chagas’ disease). This study aimed to evaluate the in vitro antioxidant and anti-glycation activities of the crude hydroethanolic extract of M. velutina xylopodium. Besides, it aimed to evaluate its effect on parasitemia levels in vivo T. cruzi experimental infection. In addition, this study aimed to determine possible interactions between the main compound of the extract and molecular targets associated with survival and virulence of T. cruzi in silico approaches. Determination of total polyphenols, flavonoids and steroidal aglycones content were performed. In addition, high performance liquid chromatography (HPLC) was carried out to identify main compounds of the extract. Antioxidant activity was determined by DPPH radical scavenging, ferric ion reducing power (FRAP), Thiobarbituric acid reactive species (TBARS) and Oxygen Radical Absorbance Capacity (ORAC) methods. Anti-glycation activity was demonstrated through relative mobility in electrophoresis (RME), determination of free amino groups and inhibition of AGEs formation. Determination of the action of extract in parasitemia levels was performed by T. cruzi experimental infection of mice and nitrite levels were measured in the serum of animals evaluated in this study. Molecular docking analyses of the main compound (Velutinol A) with DNA and molecular targets associated with survival and virulence of T. cruzi. Phytoconstituents evaluation exhibited the presence polyphenols, flavonoids and steroidal aglycone, and HPLC identified the major presence of Velutinol A. Antioxidant and anti-glycation evaluations showed that the extract present significant activity in all methods evaluated. In addition, extract reduced the number of trypomastigotes and increased the survival of treated animals. The treatment using extract showed an interference in the synthesis of physiological nitric oxide as an immune response to infection. In silico assays demonstrated interaction between Velutinol A and DNA and molecular targets of T. cruzi. The results showed that
doi_str_mv 10.1016/j.jep.2024.118994
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Ex Stadelm.) Woodson, known in Brazil as "infalível" and "jalapa", is a medicinal plant native from the Cerrado region (Brazilian Savannah). The underground organ (xylopodium) of this species is prepared as ethanolic extract or infusion and it is commonly used in traditional medicine to treat snake venom. Although, locals and indigenous populations from Cerrado have used M. velutina for the treatment of infection by Trypanosoma cruzi (Chagas’ disease). This study aimed to evaluate the in vitro antioxidant and anti-glycation activities of the crude hydroethanolic extract of M. velutina xylopodium. Besides, it aimed to evaluate its effect on parasitemia levels in vivo T. cruzi experimental infection. In addition, this study aimed to determine possible interactions between the main compound of the extract and molecular targets associated with survival and virulence of T. cruzi in silico approaches. Determination of total polyphenols, flavonoids and steroidal aglycones content were performed. In addition, high performance liquid chromatography (HPLC) was carried out to identify main compounds of the extract. Antioxidant activity was determined by DPPH radical scavenging, ferric ion reducing power (FRAP), Thiobarbituric acid reactive species (TBARS) and Oxygen Radical Absorbance Capacity (ORAC) methods. Anti-glycation activity was demonstrated through relative mobility in electrophoresis (RME), determination of free amino groups and inhibition of AGEs formation. Determination of the action of extract in parasitemia levels was performed by T. cruzi experimental infection of mice and nitrite levels were measured in the serum of animals evaluated in this study. Molecular docking analyses of the main compound (Velutinol A) with DNA and molecular targets associated with survival and virulence of T. cruzi. Phytoconstituents evaluation exhibited the presence polyphenols, flavonoids and steroidal aglycone, and HPLC identified the major presence of Velutinol A. Antioxidant and anti-glycation evaluations showed that the extract present significant activity in all methods evaluated. In addition, extract reduced the number of trypomastigotes and increased the survival of treated animals. The treatment using extract showed an interference in the synthesis of physiological nitric oxide as an immune response to infection. In silico assays demonstrated interaction between Velutinol A and DNA and molecular targets of T. cruzi. The results showed that the hydroethanolic extract of M. velutina xylopodium contains bioactive compounds including polyphenols, flavonoids and steroidal aglycones (mainly Velutinol A) of which may be responsible for the antioxidant, anti-glycation and anti-parasitic activity against T. cruzi. Trypanocidal activity of M. velutina compounds may be linked to their influence on NO synthesis during infection and/or their capacity to bind and inhibit molecules associated to virulence and survival of T. cruzi. [Display omitted] •Mandevilla velutina presents antioxidant, anti-glycation and trypanocidal potential.•M. velutina xylopodium extract contains Velutinol A as main active compound.•M. velutina xylopodium extract increased physiological NO in T. cruzi infection.•In silico showed interaction between Velutinol A and DNA or molecules of T. cruzi.</description><identifier>ISSN: 0378-8741</identifier><identifier>ISSN: 1872-7573</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2024.118994</identifier><identifier>PMID: 39461387</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; antioxidants ; Antioxidants - pharmacology ; antiparasitic properties ; blood serum ; Brazil ; cerrado ; Chagas Disease - drug therapy ; Chagas Disease - parasitology ; chemical constituents of plants ; Computer Simulation ; DNA ; electrophoresis ; flavonoids ; high performance liquid chromatography ; immune response ; Male ; Mandevilla ; medicinal plants ; Mice ; Molecular Docking Simulation ; Nitric oxide ; nitrites ; oxygen radical absorbance capacity ; parasitemia ; Parasitemia - drug therapy ; Parasites ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; polyphenols ; snake venoms ; species ; thiobarbituric acid-reactive substances ; traditional medicine ; Trypanocidal ; Trypanocidal Agents - pharmacology ; Trypanosoma cruzi ; Trypanosoma cruzi - drug effects ; Trypanosomes ; trypomastigotes ; Velutinol A ; virulence</subject><ispartof>Journal of ethnopharmacology, 2025-01, Vol.337 (Pt 2), p.118994, Article 118994</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-181ca9a19d5689e604d7031e89dc72bef23c3e4f81a3a74d7bba80e4d2a285713</cites><orcidid>0000-0002-0300-0247</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39461387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Regildo Márcio Gonçalves da</creatorcontrib><creatorcontrib>Barbosa, Fernando Cesar</creatorcontrib><creatorcontrib>Santos, Hugo Henrique</creatorcontrib><creatorcontrib>Granero, Filipe Oliveira</creatorcontrib><creatorcontrib>Figueiredo, Célia Cristina Malaguti</creatorcontrib><creatorcontrib>Nicolau-Junior, Nilson</creatorcontrib><creatorcontrib>Hamaguchi, Amélia</creatorcontrib><creatorcontrib>Silva, Luciana Pereira</creatorcontrib><title>Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Mandevilla velutina (Mart. Ex Stadelm.) Woodson, known in Brazil as "infalível" and "jalapa", is a medicinal plant native from the Cerrado region (Brazilian Savannah). The underground organ (xylopodium) of this species is prepared as ethanolic extract or infusion and it is commonly used in traditional medicine to treat snake venom. Although, locals and indigenous populations from Cerrado have used M. velutina for the treatment of infection by Trypanosoma cruzi (Chagas’ disease). This study aimed to evaluate the in vitro antioxidant and anti-glycation activities of the crude hydroethanolic extract of M. velutina xylopodium. Besides, it aimed to evaluate its effect on parasitemia levels in vivo T. cruzi experimental infection. In addition, this study aimed to determine possible interactions between the main compound of the extract and molecular targets associated with survival and virulence of T. cruzi in silico approaches. Determination of total polyphenols, flavonoids and steroidal aglycones content were performed. In addition, high performance liquid chromatography (HPLC) was carried out to identify main compounds of the extract. Antioxidant activity was determined by DPPH radical scavenging, ferric ion reducing power (FRAP), Thiobarbituric acid reactive species (TBARS) and Oxygen Radical Absorbance Capacity (ORAC) methods. Anti-glycation activity was demonstrated through relative mobility in electrophoresis (RME), determination of free amino groups and inhibition of AGEs formation. Determination of the action of extract in parasitemia levels was performed by T. cruzi experimental infection of mice and nitrite levels were measured in the serum of animals evaluated in this study. Molecular docking analyses of the main compound (Velutinol A) with DNA and molecular targets associated with survival and virulence of T. cruzi. Phytoconstituents evaluation exhibited the presence polyphenols, flavonoids and steroidal aglycone, and HPLC identified the major presence of Velutinol A. Antioxidant and anti-glycation evaluations showed that the extract present significant activity in all methods evaluated. In addition, extract reduced the number of trypomastigotes and increased the survival of treated animals. The treatment using extract showed an interference in the synthesis of physiological nitric oxide as an immune response to infection. In silico assays demonstrated interaction between Velutinol A and DNA and molecular targets of T. cruzi. The results showed that the hydroethanolic extract of M. velutina xylopodium contains bioactive compounds including polyphenols, flavonoids and steroidal aglycones (mainly Velutinol A) of which may be responsible for the antioxidant, anti-glycation and anti-parasitic activity against T. cruzi. Trypanocidal activity of M. velutina compounds may be linked to their influence on NO synthesis during infection and/or their capacity to bind and inhibit molecules associated to virulence and survival of T. cruzi. [Display omitted] •Mandevilla velutina presents antioxidant, anti-glycation and trypanocidal potential.•M. velutina xylopodium extract contains Velutinol A as main active compound.•M. velutina xylopodium extract increased physiological NO in T. cruzi infection.•In silico showed interaction between Velutinol A and DNA or molecules of T. cruzi.</description><subject>Animals</subject><subject>antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>antiparasitic properties</subject><subject>blood serum</subject><subject>Brazil</subject><subject>cerrado</subject><subject>Chagas Disease - drug therapy</subject><subject>Chagas Disease - parasitology</subject><subject>chemical constituents of plants</subject><subject>Computer Simulation</subject><subject>DNA</subject><subject>electrophoresis</subject><subject>flavonoids</subject><subject>high performance liquid chromatography</subject><subject>immune response</subject><subject>Male</subject><subject>Mandevilla</subject><subject>medicinal plants</subject><subject>Mice</subject><subject>Molecular Docking Simulation</subject><subject>Nitric oxide</subject><subject>nitrites</subject><subject>oxygen radical absorbance capacity</subject><subject>parasitemia</subject><subject>Parasitemia - drug therapy</subject><subject>Parasites</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>polyphenols</subject><subject>snake venoms</subject><subject>species</subject><subject>thiobarbituric acid-reactive substances</subject><subject>traditional medicine</subject><subject>Trypanocidal</subject><subject>Trypanocidal Agents - pharmacology</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - drug effects</subject><subject>Trypanosomes</subject><subject>trypomastigotes</subject><subject>Velutinol A</subject><subject>virulence</subject><issn>0378-8741</issn><issn>1872-7573</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><recordid>eNqNkUtv1DAUhSMEokPhB7BBXrJoBt-87MCqqnhUKmJT1tYd5wY8SpxgO1GHf8c_406nsEQsLL--c650Tpa9BLkFCc2b_XZP87aQRbUF0G1bPco2oFWRq1qVj7ONLJXOtargLHsW415KqaCST7Ozsq0aKLXaZL8ufXLTnevQJ4G-45Vc_m04WOR3L9Amt7rkKIqpF5-ZoNUNA4qVhiU5j4LuUmDqXkx9T3xk3YwBo0s0OhQDMRyF8-I2HGb0U5xGFDYsPx2rZwpuJJ9wYOIo57FvxbUXq1uni6OK54fp3p8v0Q3O8m2ew4T2O8Xn2ZMeh0gvHvbz7OuH97dXn_KbLx-vry5vcls0OuWgwWKL0HZ1o1tqZNUpWQLptrOq2FFflLakqteAJSr-3O1QS6q6AgtdKyjPs9cnXx78Y6GYzOiiJc7C07REU0Jdga5r0P-BFlAoBXXDKJxQG6YYA_Vm5jgwHAxIcyzZ7A2XbI4lm1PJrHn1YL_sRur-Kv60ysC7E8Cxc10UTLSOvKXOBQ7YdJP7h_1vm668GA</recordid><startdate>20250130</startdate><enddate>20250130</enddate><creator>Silva, Regildo Márcio Gonçalves da</creator><creator>Barbosa, Fernando Cesar</creator><creator>Santos, Hugo Henrique</creator><creator>Granero, Filipe Oliveira</creator><creator>Figueiredo, Célia Cristina Malaguti</creator><creator>Nicolau-Junior, Nilson</creator><creator>Hamaguchi, Amélia</creator><creator>Silva, Luciana Pereira</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-0300-0247</orcidid></search><sort><creationdate>20250130</creationdate><title>Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches</title><author>Silva, Regildo Márcio Gonçalves da ; 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Ex Stadelm.) Woodson, known in Brazil as "infalível" and "jalapa", is a medicinal plant native from the Cerrado region (Brazilian Savannah). The underground organ (xylopodium) of this species is prepared as ethanolic extract or infusion and it is commonly used in traditional medicine to treat snake venom. Although, locals and indigenous populations from Cerrado have used M. velutina for the treatment of infection by Trypanosoma cruzi (Chagas’ disease). This study aimed to evaluate the in vitro antioxidant and anti-glycation activities of the crude hydroethanolic extract of M. velutina xylopodium. Besides, it aimed to evaluate its effect on parasitemia levels in vivo T. cruzi experimental infection. In addition, this study aimed to determine possible interactions between the main compound of the extract and molecular targets associated with survival and virulence of T. cruzi in silico approaches. Determination of total polyphenols, flavonoids and steroidal aglycones content were performed. In addition, high performance liquid chromatography (HPLC) was carried out to identify main compounds of the extract. Antioxidant activity was determined by DPPH radical scavenging, ferric ion reducing power (FRAP), Thiobarbituric acid reactive species (TBARS) and Oxygen Radical Absorbance Capacity (ORAC) methods. Anti-glycation activity was demonstrated through relative mobility in electrophoresis (RME), determination of free amino groups and inhibition of AGEs formation. Determination of the action of extract in parasitemia levels was performed by T. cruzi experimental infection of mice and nitrite levels were measured in the serum of animals evaluated in this study. Molecular docking analyses of the main compound (Velutinol A) with DNA and molecular targets associated with survival and virulence of T. cruzi. Phytoconstituents evaluation exhibited the presence polyphenols, flavonoids and steroidal aglycone, and HPLC identified the major presence of Velutinol A. Antioxidant and anti-glycation evaluations showed that the extract present significant activity in all methods evaluated. In addition, extract reduced the number of trypomastigotes and increased the survival of treated animals. The treatment using extract showed an interference in the synthesis of physiological nitric oxide as an immune response to infection. In silico assays demonstrated interaction between Velutinol A and DNA and molecular targets of T. cruzi. The results showed that the hydroethanolic extract of M. velutina xylopodium contains bioactive compounds including polyphenols, flavonoids and steroidal aglycones (mainly Velutinol A) of which may be responsible for the antioxidant, anti-glycation and anti-parasitic activity against T. cruzi. Trypanocidal activity of M. velutina compounds may be linked to their influence on NO synthesis during infection and/or their capacity to bind and inhibit molecules associated to virulence and survival of T. cruzi. [Display omitted] •Mandevilla velutina presents antioxidant, anti-glycation and trypanocidal potential.•M. velutina xylopodium extract contains Velutinol A as main active compound.•M. velutina xylopodium extract increased physiological NO in T. cruzi infection.•In silico showed interaction between Velutinol A and DNA or molecules of T. cruzi.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>39461387</pmid><doi>10.1016/j.jep.2024.118994</doi><orcidid>https://orcid.org/0000-0002-0300-0247</orcidid></addata></record>
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identifier ISSN: 0378-8741
ispartof Journal of ethnopharmacology, 2025-01, Vol.337 (Pt 2), p.118994, Article 118994
issn 0378-8741
1872-7573
1872-7573
language eng
recordid cdi_proquest_miscellaneous_3154185518
source ScienceDirect Freedom Collection
subjects Animals
antioxidants
Antioxidants - pharmacology
antiparasitic properties
blood serum
Brazil
cerrado
Chagas Disease - drug therapy
Chagas Disease - parasitology
chemical constituents of plants
Computer Simulation
DNA
electrophoresis
flavonoids
high performance liquid chromatography
immune response
Male
Mandevilla
medicinal plants
Mice
Molecular Docking Simulation
Nitric oxide
nitrites
oxygen radical absorbance capacity
parasitemia
Parasitemia - drug therapy
Parasites
Plant Extracts - chemistry
Plant Extracts - pharmacology
polyphenols
snake venoms
species
thiobarbituric acid-reactive substances
traditional medicine
Trypanocidal
Trypanocidal Agents - pharmacology
Trypanosoma cruzi
Trypanosoma cruzi - drug effects
Trypanosomes
trypomastigotes
Velutinol A
virulence
title Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches
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