Three RNA helicase DDX genes are essential for the development and oocyte maturation in Laodelphax striatellus

• Published in: Pest management science 2024-12, Vol.80 (12), p.6575-6584
• Main Authors: Ma, Qing‐Lu, Zhang, Chuan‐Xi, Chen, Jian‐Ping, Li, Jun‐Min, Zhang, Yan
• Format: Article
• Language: English
• Subjects: Animals; carcinogenesis; Cell cycle; DEAD-box RNA helicases; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; DEAD‐box helicase; Developmental stages; DNA helicase; family; Female; Fertility; Functionals; Gametocytes; Genes; Hemiptera - enzymology; Hemiptera - genetics; Hemiptera - growth & development; Hemiptera - physiology; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Laodelphax striatellus; metabolism; Nymph - genetics; Nymph - growth & development; oocytes; Oocytes - metabolism; ovarian development; Ovaries; Pest control; Pests; phenotype; Phenotypes; Phylogeny; Plant viruses; Protein turnover; reproduction; Ribonucleic acid; rice; RNA; RNA helicase; RNA interference; RNA-mediated interference; sap; signal transduction; Structural analysis; Survival; survival rate; Tumorigenesis
• ISSN: 1526-498X; 1526-4998; 1526-4998
• DOI: 10.1002/ps.8396

Background DEAD‐box protein (DDX) is a member of the DDX RNA helicase family that exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility, particularly in mammals. Nevertheless, the biological functions of DDXs in insects have not been fully resolved and attracted increasing attention these years. Laodelphax striatellus (Hemiptera) is a notorious rice pest through feeding on rice sap and transmitting plant viruses. In this study, we aim to elucidate the functional characterization of DDXs in L. striatellus, and to exploit potential target genes for the development of pest control strategies. Results In this study, we characterized the expression patterns of LsDDX6, LsDDX47, and LsDDX51 in planthoppers and analyzed their conserved motifs. These genes were found to be expressed in all tissues and developmental stages examined, with significantly higher transcript levels observed in the ovary. Knockdown of LsDDX6, LsDDX47, and LsDDX51 resulted in an obvious lethal phenotype in nymphs and abnormal ovarian development in adults. Furthermore, a total of 27 DDXs were identified in L. striatellus, and most DDXs were highly expressed in ovary and structure analysis result revealed that all of the DDXs possessed nine motifs that were unique to the DDX family. Conclusion The three DDX RNA helicases (LsDDX6, LsDDX47, and LsDDX51) are essential for both survivorship and reproduction in L. striatellus. Considering a total number of 27 DDXs identified in L. striatellus, they might serve as promising candidates for application in RNAi‐based control of this destructive pest. © 2024 Society of Chemical Industry. LsDDX6, LsDDX47, and LsDDX51 are essential to maintaining life and ovarian development in L. striatellus, indicating they could be a potential targets for pest control.