Structural characterization of a sulfated polysaccharide from Gracilariadomingensis and potential anti-inflammatory and antinociceptive effects

• Published in: Carbohydrate research 2025-01, Vol.547, p.109322, Article 109322
• Main Authors: da Silva Prudêncio, Rafael, Sousa, Antonio Kleiton de, Silva, Denise Mayara Melo, Santos Ferreira, Jayro dos, Rocha, Danyela Maria Leal, Almeida, Viviane Pinheiro Alves de, Silva Sousa, João Janilson da, Sousa, Stefany Guimarães, da Silva, Tino Marcos Lino, dos Santos Carvalho, André, Cruz Júnior, José Simião da, Alves, Even Herlany Pereira, Vasconcelos, Daniel Fernando Pereira, Bezerra, Roosevelt D.S., França Dourado, Flaviane de, Oliveira, Aline Lima de, Cabral, Wanessa Feliz, Souza de Almeida Leite, José Roberto de, da Silva, Durcilene Alves, Nascimento Junior, Elias Borges do, Brito, Tarcísio Vieira de, Aguiar Magalhães, Diva de, Barbosa, André Luiz dos Reis
• Format: Article
• Language: English
• Subjects: acetic acid; Analgesics - chemistry; Analgesics - pharmacology; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Biological activity; biopolymers; bradykinin; Carrageenan; Cell migration; cell movement; cytokines; Edema; Edema - chemically induced; Edema - drug therapy; formalin; glutathione; Gracilaria - chemistry; Gracilaria domingensis; histamine; Inflammation; Male; malondialdehyde; Marine polysaccharides; Mice; myeloperoxidase; neutrophils; pain; peritonitis; Peritonitis - chemically induced; Peritonitis - drug therapy; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; prostaglandins; Rats; serotonin; Sulfates - chemistry; Sulfates - pharmacology
• ISSN: 0008-6215; 1873-426X; 1873-426X
• DOI: 10.1016/j.carres.2024.109322

Seaweeds are natural sources of sulfated polysaccharides (SPs), biopolymers with remarkable pharmacological properties, including biological actions capable of attenuating components of the inflammatory process such as edema, cytokines, cell migration and pain. Our results confirm that SPs obtained from Gracilaria domingensis (SP-GD) are agarans, primarily composed of residues of β-d-galactopyranose 6-sulfate and 3,6-anhydro-α-l-galactopyranose. Specifically, SP-GD at a dose of 10 mg/kg was effective in significantly reducing paw edema induced by carrageenan or histamine, serotonin, bradykinin, 48/80 and prostaglandin E2. SP-GD (10 mg/kg) was also able to reduce neutrophil migration and the activity of the myeloperoxidase enzyme in carrageenan-induced peritonitis, as well as conserve glutathione concentration and reduce malondialdehyde levels in the animals' peritoneal fluid. Furthermore, it showed antinociceptive action in the abdominal writhing test induced by acetic acid and in the paw licking test induced by formalin. Thus, the results obtained allow us to infer that SPs extracted from G. domingensis at a dose of 10 mg/kg have anti-inflammatory effects by reducing neutrophil migration and modulating the activity of vasoactive mediators and antinociceptive effects by acting, at least in part, through a peripheral mechanism dependent on the negative modulation of inflammatory mediators. [Display omitted] •There is increasing interest in marine algae polysaccharides.•The biological activities of sulfate polysaccharides were confirmed.•Pharmacological interest in natural polysaccharides has increased.•There are broad prospects for applications of natural seaweed polysaccharides.