One-step construction of bioinspired multi-enzyme mimicking nanozyme as a universal platform for multi-mode sensing and catalytic degradation

• Published in: Biosensors & bioelectronics 2025-02, Vol.270, p.116991, Article 116991
• Main Authors: Zhang, Yuqi, Zhang, Kaidi, Yao, Liying, Dong, Jiamin, Li, Peiqi, Wang, Yuxin, Daka, Zamar, Zheng, Yang, Liu, Wenyuan, Ji, Shunli
• Format: Article
• Language: English
• Subjects: active sites; Bioinspired; Biomimetic Materials - chemistry; biomimetics; Biosensing Techniques - methods; biosensors; Catalysis; Copper - chemistry; cost effectiveness; Environmental remediation; epinephrine; glutathione; Glutathione - analysis; Glutathione - chemistry; Green catalysis; laccase; Laccase - chemistry; melamine; Multi-enzyme mimicking nanozyme; nanomaterials; Nanostructures - chemistry; remediation; sulfides; Sulfides - chemistry; Triazines - analysis; Triazines - chemistry; Versatile sensing platform
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2024.116991

Nanozymes, a category of nanomaterials with exceptional enzyme-like activity, exhibit the significant promise to overcome the inherent limitations of natural enzymes. Inspired by the active site structure of natural laccase, a biomimetic MA-Cu nanozyme with three-dimensional network structure was constructed in water system through one-step complexation based on the specific coordination between nitrogen-rich triazine heterocyclic melamine and Cu2+, in a facile, green and economical manner. Compared to natural laccase, MA-Cu possesses superior multi-enzyme mimicking activity, stability and cost-effectiveness. Through comprehensive characterizations, activity tests and theoretical calculations, the catalytic mechanism and the ligand-tunability of enzyme-like activity have been thoroughly investigated. Based on its multi-enzyme-like activities, a multifunctional monitoring platform for sulfide in food, epinephrine in preparations and glutathione in cells was successfully constructed, respectively. Notably, a green degradation and discrimination platform based on MA-Cu for various pollutants was developed, exhibiting distinguished substrate universality and detoxication capacity. As a stable, easily scalable and commercially applicable nanozyme, MA-Cu is expected to become a compelling candidate for replacing natural enzyme, showing excellent prospects in environmental remediation and biosensing. [Display omitted]