A novel cell wall galactofuranan in Clavibacter phaseoli VKM Ac-2641T

• Published in: Carbohydrate research 2023-03, Vol.525, p.108778-108778, Article 108778
• Main Authors: Perepelov, Andrey V., Kim, Deborah, Tul'skaya, Elena M., Potekhina, Nataliya V., Dmitrenok, Andrey S., Senchenkova, Sof'ya N., Dorofeeva, Lubov V., Evtushenko, Lyudmila I., Shashkov, Alexander S.
• Format: Article
• Language: English
• Subjects: Cell wall glycopolymer; cell walls; class; Clavibacter; family; Galactofuranan; plant pathogens; spectroscopy; Taxonomy; trisaccharides
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2023.108778

A glycopolymer of novel structure was found in the cell wall of plant pathogen Clavibacter phaseoli VKM Ac-2641T (family Microbacteriaceae, class Actinomycetes). The glycopolymer was (1 → 6)-linked β-d-galactofuranan with side branched trisaccharide, α-D-Manp-(1 → 2)-[α-D-Manp-(1 → 3)]-α-D-Ribf-(1→ at O-2 on every second galactofuranose residue. The galactofuranan structure was established by chemical and NMR spectroscopic methods using one- and two-dimensional techniques 1H,1H COSY, TOCSY, ROESY and 1H,13C HSQC, HMBC. The results of this study provide new data on diversity of bacterial glycopolymers, may prove useful for bacterial taxonomy and contribute to the understanding of the host plant-microbiota interaction mechanisms. [Display omitted] •A galactofuranan of novel structure is identified in C. phaseoli VKM Ac-2641T.•The (1. → 6)-linked galactofuranan has side trisaccharide at O-2 on every second Galf.•The side trisaccharide consists of Ribf, substituted by two Manp residues at O-2,3.