Simultaneous Quantitation of Bioactive Compounds in Different Varieties of Arachis Hypogea L. Using Liquid Chromatography/ESI Mass Spectrometry: A Chemometric Approach

Cultivated Arachis hypogaea L., is commonly known as peanut. Its seed coat, also referred to as a shell, plays a critical role in safeguarding the seeds and facilitating the transportation of diverse metabolites. In the current work, an examination of metabolite profiling was executed utilizing UPLC...

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Bibliographic Details
Published in:Chromatographia 2024-12, Vol.87 (11-12), p.773-784
Main Authors: Nandan, Shiv, Khan, Mohd Amish, Khan, Mohsin Ali, Shukla, Vijaya, Srivastava, Madhumita, Khan, Mohammad Faheem
Format: Article
Language:English
Subjects:
Analytical Chemistry
Arachis hypogaea
Chemistry
Chemistry and Materials Science
chemometrics
Chromatography
detection limit
Geographical locations
Impact analysis
India
Laboratory Medicine
Liquid chromatography
luteolin
Mass spectrometry
Metabolites
Original
Peanuts
Pharmacy
principal component analysis
Principal components analysis
Proteomics
pulp
rutin
seed coat
standard deviation
transportation
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Summary:Cultivated Arachis hypogaea L., is commonly known as peanut. Its seed coat, also referred to as a shell, plays a critical role in safeguarding the seeds and facilitating the transportation of diverse metabolites. In the current work, an examination of metabolite profiling was executed utilizing UPLC-ESI–MS/MS in the seed coat (SeC), seed skin (SeS), and seed pulp (SeP) of four distinct peanut varieties of different geographical locations in India. The method was also validated according to ICH guidelines. A linear detector response was observed within a concentration range of 0.019–20.0 ng/mL, R 2  = 0.999. The limit of detection (LOD) and quantification (LOQ) of the targeted compounds were found to be within the range of 0.075–0.426 and 0.227–1.292 ng/mL, respectively. The intra-day and inter-day precision demonstrated relative standard deviation %RSD 
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-024-04368-2