New lactate- and pyruvate-containing polysaccharide and rhamnomannan with xylose residues from the cell wall of Rathayibacter oskolensis VKM Ac-2121T

• Published in: Carbohydrate research 2024-06, Vol.540, p.109145-109145, Article 109145
• Main Authors: Shashkov, Alexander S., Potekhina, Natalia V., Tul'skaya, Elena M., Dmitrenok, Andrey S., Senchenkova, Sof'ya N., Torgov, Vladimir I., Dorofeeva, Lubov V., Evtushenko, Lyudmila I.
• Format: Article
• Language: English
• Subjects: Cell wall glycopolymers; cell walls; class; endophytes; family; genus; Lactate; lactic acid; polymers; polysaccharides; Pyruvate; pyruvic acid; Rathayibacter; Rathayibacter oskolensis; Rhamnomannan; Rhamnose-containing polysaccharides; spectroscopy; xylose
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2024.109145

The cell wall of endophytic strain Rathayibacter oskolensis VKM Ac-2121T (family Microbacteriaceae, class Actinomycetes) was found to contain neutral and acidic glycopolymers. The neutral polymer is a block-type rhamnomannan partially should be substitutied by xylose residues, [→2)-α-[β-D-Xylp-(1 → 3)]-D-Manp-(1 → 3)-α-D-Rhap-(1→]∼30 [→2)-α-D-Manp-(1 → 3)-α-D-Rhap-(1→]∼45. The acidic polymer has branched chain, bearing lactate and pyruvate residues, →4)-α-D-[S-Lac-(2–3)-α-L-Rhap-(1 → 3)]-D-Manp-(1 → 3)-α-D-[4,6-R-Pyr]-D-Galp-(1 → 3)-β-D-Glcp-(1 →. The structures of both glycopolymers were not described in the Gram-positive bacteria to date. The glycopolymers were studied by chemical and NMR spectroscopic methods. The results of this study provide new data on diversity of bacterial glycopolymers and may prove useful in the taxonomy of the genus Rathayibacter and for understanding the molecular mechanisms of interaction between plants and plant endophytes. [Display omitted] •New structures of neutral and acidic polysaccharides are presented.•Neutral polymer is a block-type rhamnomannan partially substituted by Xylp residues.•The acidic polymer is branched lactate- and pyruvate-containing polysaccharide.