Ultrasound-laccase pre-treatment enhances agarwood essential oil extraction and bioactivity

• Published in: International journal of biological macromolecules 2025-03, Vol.295, p.139654, Article 139654
• Main Authors: Ma, Sheng, Chen, Yuan, Yan, Tingting, Qin, Jiahui, Li, Gaiyun
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - chemistry; Acetylcholinesterase - metabolism; Acetylcholinesterase inhibition; Agarwood essential oil; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cholinesterase Inhibitors - chemistry; Cholinesterase Inhibitors - isolation & purification; Cholinesterase Inhibitors - pharmacology; Glycoside Hydrolase Inhibitors - chemistry; Glycoside Hydrolase Inhibitors - isolation & purification; Glycoside Hydrolase Inhibitors - pharmacology; Oils, Volatile - chemistry; Oils, Volatile - pharmacology; Sesquiterpene oxides; Sustainable extraction; Thymelaeaceae - chemistry; Ultrasonic Waves; Ultrasound-assisted laccase; Wood - chemistry; α-Glucosidase inhibition
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2025.139654

Agarwood essential oil is prized for its elegant aroma and pharmacological properties; however, the traditional hydrodistillation method suffers from inefficiencies, constraining the industrial potential of agarwood. We proposed an ultrasonic-assisted laccase synergistic pretreatment technique that enhanced extraction throughput by 70.90 % compared to the traditional method by facilitating pore formation in agarwood and expediting the release of essential oil. The essential oil extracted using this method retained a similar aromatic profile to the traditional method but achieved a higher sesquiterpene oxide concentration (95.3 % vs. 89.6 %). Additionally, it exhibited notable improvements in antioxidant activity, acetylcholinesterase inhibition, and α-glucosidase inhibition, attributed to the increased affinity of sesquiterpene oxides for enzymes through hydrogen bonding, covalent interactions, and hydrophobic effects with amino acid residues. This approach not only maximises the efficiency of agarwood essential oil extraction and amplifies its bioactive properties, but also offers a sustainable and eco-friendly alternative, promoting the long-term vitality of the agarwood industry. [Display omitted] •Ultrasound-laccase pre-treatment significantly enhances the yield of agarwood essential oil.•The agarwood essential oil's sesquiterpene oxide content was increased by ultrasound-laccase pre-treatment.•The increased sesquiterpene oxides of agarwood essential oils significantly enhance its bioactivity.