Anti-inflammatory, immunostimulant and antimicrobial properties of tannic acid in the diet of Oreochromis niloticus infected with Aeromonas hydrophila

• Published in: Fish & shellfish immunology 2025-02, Vol.157, p.110119, Article 110119
• Main Authors: Miguel Libanori, Maria Clara, Gomes dos Santos, Gracienhe, Souza, Ana Paula, da Silva Costa, Domickson, Saldaña-Serrano, Miguel, Ferreira, Matheus Berlofa, de Araújo Pereira Junior, Jucimauro, Bellettini, Frank, Pereira Dutra, Scheila Anelise, Martins, Maurício Laterça, Owatari, Marco Shizuo, Pedreira Mouriño, José Luiz
• Format: Article
• Language: English
• Subjects: Feed strategy; Fish immunity; Functional additive; Sanity; Tilapia health
• ISSN: 1050-4648; 1095-9947; 1095-9947
• DOI: 10.1016/j.fsi.2025.110119

The study aimed to assess the impact of dietary supplementation with tannic acid on the growth, health, and survival of Oreochromis niloticus following exposure to Aeromonas hydrophila. A total of 320 fish were divided into 16 tanks and assigned to four treatment groups: feed with 0.2 % tannic acid (TA0.2 %), 0.4 % tannic acid (TA0.4 %), 0.8 % tannic acid (TA0.8 %), or no tannic acid (Control0%), with each treatment replicated four times, over a 50-day period. At the end of the 50-day period, biological samples were collected from the fish, which were then intraperitoneally injected with A. hydrophila. No significant differences in growth performance were detected between treatments. As expected, levels of total leukocytes, lymphocytes, monocytes, hemoglobin, and mean corpuscular hemoglobin concentration (MCHC) were notably higher in the blood of the fish after infection, regardless of the treatment received. During both the pre- and post-infection periods, monocytes were more abundant in the TA0.2 % and TA0.8 % treatments compared to the TA0.4 % treatment. Additionally, there was a significant interaction between the factors affecting thrombocytes, neutrophils, basophils, hemoglobin, and MCHC. Thrombocytosis and neutrophilia were significantly greater in the TA0.8 % treatment pre-infection than in the post-infection and control group. Conversely, a higher number of basophils were observed in the post-infection period in the TA0.8 % treatment group compared to the pre-infection period. Total plasma protein levels decreased significantly in the post-infection period, regardless of tannic acid supplementation levels, while immunoglobulin levels increased after exposure to A. hydrophila. Histological analyses revealed a significant increase in the perimeter and number of intestinal villi in the TA0.4 % treatment group before infection. The number of goblet cells also increased in the control group (0 %), TA0.4 %, and TA0.8 % before infection. In splenic tissue, the TA0.4 % treatment resulted in a reduction in eosinophilic and mononuclear infiltrates, as well as decreased congestion and vacuolation. Hemosiderin levels were lower in the TA0.4 % and TA0.2 % treatment groups. In the liver, lymphocytic infiltrates were reduced in the TA0.2 % and TA0.4 % treatment groups, and portal vein congestion was decreased in the TA0.2 % post-infection and TA0.4 % pre-infection groups. Post-infection survival rates were significantly higher (p