Assembling branched and macrocyclic peptides on proteins

A two-step, biocompatible strategy enables site-specific generation of branched and macrocyclic peptide-protein conjugates. Solvent-exposed cysteines on proteins are modified by a small bifunctional reagent at near-physiological pH, followed by cyanopyridine-aminothiol click reactions to create bran...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2025-02, Vol.61 (14), p.3009-3012
Main Authors: Ullrich, Sven, Kumaresan, Santhanalaxmi, Rahman, Marina G, Panda, Bishvanwesha, Morewood, Richard, Nitsche, Christoph
Format: Article
Language:English
Subjects:
Biocompatibility
Click Chemistry - methods
Cysteine - chemistry
Peptides
Peptides, Cyclic - chemical synthesis
Proteins
Proteins - chemistry
Proteins - therapeutic use
Pyridines - chemistry
Reagents
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Summary:A two-step, biocompatible strategy enables site-specific generation of branched and macrocyclic peptide-protein conjugates. Solvent-exposed cysteines on proteins are modified by a small bifunctional reagent at near-physiological pH, followed by cyanopyridine-aminothiol click reactions to create branched or macrocyclic peptide architectures. This method offers design strategies for next-generation protein therapeutics.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d4cc06442a