Studies on protein adsorption in a vortex flow reactor

The performance of a vortex flow reactor (VFR) with suspended particles for protein adsorption was studied under varying operating conditions, and resin volume fractions. The VFR behaved as an expanded bed in the regimen of laminar vortices flow. Streamline DEAE was used for bovine serum albumin (BS...

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Bibliographic Details
Published in:Process biochemistry (1991) 2007-12, Vol.42 (12), p.1592-1601
Main Authors: Ibáñez-González, Maria J., Cooney, Charles L.
Format: Article
Language:English
Subjects:
Expanded bed
Heterogeneous reactor
Protein adsorption
Protein purification
Vortex flow reactor
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Summary:The performance of a vortex flow reactor (VFR) with suspended particles for protein adsorption was studied under varying operating conditions, and resin volume fractions. The VFR behaved as an expanded bed in the regimen of laminar vortices flow. Streamline DEAE was used for bovine serum albumin (BSA) adsorption. Expanded bed VFR experiments were performed with varying geometric aspect ratios (14.6, 28.6 and 40.0) and axial superficial velocity (100–300 cm h −1) to investigate their influence on productivity and dynamic capacity. The results are compared with literature data on an expanded bed column (EBC). Adsorption breakthrough curves were fitting to a simple two-parameter model.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2007.08.012