Mitochondrial respiration in white adipose tissue is dependent on body mass index and tissue location in patients undergoing oncological or parietal digestive surgery

Adipose tissue (AT), is a major endocrine organ that plays a key role in health and disease. However, adipose dysfunctions, especially altered energy metabolism, have been under‐investigated as white adipocytes have relatively low mitochondrial density. Nevertheless, recent studies suggest that mito...

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Bibliographic Details
Published in:The FASEB journal 2025-01, Vol.39 (2), p.e70350-n/a
Main Authors: Guerrier, Lisa, Bacoeur‐Ouzillou, Ophélie, Touron, Julianne, Mezher, Sami, Cassagnes, Lucie, Vieille‐Marchiset, Aurélie, Chanon, Stéphanie, Pereira, Bruno, Pezet, Denis, Pinel, Alexandre, Gagnière, Johan, Malpuech‐Brugère, Corinne, Richard, Ruddy
Format: Article
Language:English
Subjects:
Adipose Tissue, White - metabolism
Adult
Aged
body composition
Body Mass Index
Cell Respiration - physiology
Female
human
Humans
Intra-Abdominal Fat - metabolism
lipid metabolism
Lipid Metabolism - physiology
Male
Middle Aged
Mitochondria - metabolism
mitochondrial respiration
Oxidative Phosphorylation
white adipose tissue
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Summary:Adipose tissue (AT), is a major endocrine organ that plays a key role in health and disease. However, adipose dysfunctions, especially altered energy metabolism, have been under‐investigated as white adipocytes have relatively low mitochondrial density. Nevertheless, recent studies suggest that mitochondria could play a major role in AT disorders and that AT mitochondrial activity could depend on adiposity level and location. This clinical study aimed to evaluate mitochondrial respiration and metabolism in human visceral (vAT) and subcutaneous (scAT) AT and their relationship with body mass index (BMI). This clinical study enrolled 67 patients (30 females/37 males) scheduled for digestive surgery without chemotherapy and parietal infection. BMI ranged from 15.4 to 51.9 kg·m−2 and body composition was estimated by computed tomographic images. Mitochondrial respiration was measured in situ in digitonin‐permeabilized AT using high‐resolution respirometry and a substrate/inhibitor titration approach. Protein levels of mitochondrial and lipid metabolism key elements were evaluated by Western blot. Maximal mitochondrial respiration correlated negatively with BMI (p 
ISSN:0892-6638
1530-6860
1530-6860
DOI:10.1096/fj.202402243R