Trimethylamine oxide promotes myocardial fibrosis through activating JAK2-STAT3 pathway

• Published in: Biochemical and biophysical research communications 2025-01, Vol.750, p.151390, Article 151390
• Main Authors: Yang, Xiaoyun, Wang, Yi, Feng, Yujin, Wang, Feng, Gao, Lijuan, Wang, Yueheng
• Format: Article
• Language: English
• Subjects: JAK2/STAT3; Myocardial fibrosis; TMAO
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2025.151390

Myocardial infarction is often accompanied by symptoms of myocardial fibrosis, causing myocardial systolic and diastolic dysfunction. High levels of TMAO are considered to be strongly connected to cardiovascular diseases occurrence. The objective of this investigation was to determine whether formal TMAO affects the development of cardiac muscle fibers. The left anterior descending (LAD) coronary artery was ligated to construct myocardial infarction mouse model , while the control group mouse was only operated vessel separation. According to whether or not TMAO intervention is given, mice were divided into control, TMAO, myocardial infarction model(model), and myocardial infarction model + TMAO(model+ TMAO). TMAO and model+ TMAO group received 0.24 % TMAO in drinking water for one month. After modeling, echocardiography assessed cardiac function, and cardiac tissues were collected for analysis. Masson staining observed collagen deposition, while immunohistochemistry was conducted to identify fibronectin, collagen III, collagen I, and JAK/STAT pathway related proteins levels. In vitro, mouse cardiac fibroblasts were cultured and treated with varying TMAO concentrations (0, 300, 600, 900μM), and Western blot was carried out to analyse changes in fibronectin, collagen III, and collagen I. Subsequently, the JAK2/STAT3 pathway inhibitor AG490 was utilized to treat cells and further examine the changes of fibronectin and collagen. Thein vivodata showed TMAO significantly increases fibrosis and upregulates fibronectin, collagen III, and collagen I.In vitroexperiments indicated that TMAO promotes fibronectin, collagen III, and collagen I levels through regulating the JAK2/STAT3 pathway, ultimately accelerating fibrosis. •Myocardial infarction is often accompanied by symptoms of myocardial fibrosis causing myocardial systolic and diastolic dysfunction.•Trimethylamine oxide is one of the important mechanisms of myocardial fibrosis.•Trimethylamine oxide promotes myocardial fibrosis through JAK2/STAT3 pathway.