A kinetics study on the biodegradation of synthetic wastewater simulating effluent from an advanced oxidation process using Pseudomonas putida CECT 324

• Published in: Journal of hazardous materials 2008-03, Vol.151 (2), p.780-788
• Main Authors: BALLESTEROS MARTIN, M. M, SANCHEZ PEREZ, J. A, ACIEN FERNANDEZ, F. G, GARCIA SANCHEZ, J. L, CASAS LOPEZ, J. L, MALATO RODRIGUEZ, S
• Format: Article
• Language: English
• Subjects: Advanced oxidation process; Applied sciences; Aromatic compounds; Biodegradation kinetics; Biodegradation, Environmental; Biological and medical sciences; Bioreactors; Biotechnology; Carboxylic acids; Carboxylic Acids - chemistry; Chemical engineering; Equipment Design; Exact sciences and technology; Fermentation; Fundamental and applied biological sciences. Psychology; General purification processes; Kinetics; Methods. Procedures. Technologies; Mixing; Models, Statistical; Others; Oxygen - chemistry; Pollution; Pseudomonas putida; Pseudomonas putida - metabolism; Pseudomonas putida CECT 324; Reactors; Temperature; Time Factors; Various methods and equipments; Waste Disposal, Fluid - methods; Wastewaters; Water Pollutants, Chemical - analysis; Water Purification - methods; Water treatment and pollution
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2007.06.053

Bacterial growth on mixed substrates is employed for wastewater treatment. Biodegradation kinetics of Pseudomonas putida CECT 324 growth on formic acid, vanillin, phenol and oxalic acid mixtures is described. The experiments were carried out in a stirred-tank fermentor in batch mode at different temperatures (25, 30 and 35 °C) and pH (5, 6 and 7). The four compounds selected are typical intermediates in pesticide-contaminated water treated by advanced oxidation processes (AOPs). The toxicity of intermediates was investigated for a combined AOP-biological treatment, and the minimum DOC inhibitory concentration of the intermediate mixture was 175 ppm. The resulting biodegradation and growth kinetics were best described by the sum kinetics with interaction parameters (SKIP) model. Phenol and oxalic acid inhibit P. putida growth, and formic acid consumption strongly affects the biodegradation of oxalic acid. At all the temperatures tested and at pH between 5 and 7, P. putida CECT 324 was able to degrade the four substrates after culture times of 30 h at 30 °C and pH 7, which were the best conditions, and after 70 h, under the worst, at 35 °C.