Characterization of DNA Layers Adsorbed on Glassy Carbon Electrodes

A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsome...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2008-04, Vol.20 (7), p.739-749
Main Authors: Pedano, María L., Pietrasanta, Lía I., Teijelo, Manuel López, Rivas, Gustavo A.
Format: Article
Language:English
Subjects:
AFM
DNA adsorption
DNA-modified electrode
Ellipsometry
Glassy carbon electrode
SEM
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Summary:A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsometry was also useful to evaluate the influence of the oxide layer formed on glassy carbon electrodes, either spontaneously or after electrochemical pretreatments, on the DNA adsorption and further electrooxidation process. SEM and AFM images of the electrode surface covered by a thick layer of DNA reveal a nonuniform distribution, leaving channels and islands of the biological material.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200704135