Effect of a fungal immunomodulatory protein from Ganoderma tsugae on cell cycle and interferon-gamma production through phosphatidylinositol 3-kinase signal pathway

A fungal immunomodulatory protein (FIP-gts) was purified from Ganoderma tsugae. Recombinant FIP-gts was expressed as glutathione S-transferase fusion protein in E. coli. In this study, recombinant FIP-gts promoted cell cycle progression from G0/G1 to S phase and signaling pathways were investigated...

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Bibliographic Details
Published in:Process biochemistry (1991) 2008-04, Vol.43 (4), p.423-430
Main Authors: Hsiao, Yi-Min, Huang, Yu-Lu, Tang, Sheau-Chung, Shieh, Gow-Jen, Lai, Jing-Ying, Wang, Po-Hui, Ying, Tsung-Ho, Ko, Jiunn-Liang
Format: Article
Language:English
Subjects:
Escherichia coli
Fungal immunomodulatory protein
Ganoderma
IFN-γ
PI 3-kinase
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Summary:A fungal immunomodulatory protein (FIP-gts) was purified from Ganoderma tsugae. Recombinant FIP-gts was expressed as glutathione S-transferase fusion protein in E. coli. In this study, recombinant FIP-gts promoted cell cycle progression from G0/G1 to S phase and signaling pathways were investigated in human peripheral mononuclear cells (HPBMCs) by PI staining and flow cytometry. FIP-gts significantly induced cytokine interferon-gamma (IFN-γ) expression. Pre-treatment of cells with phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor LY294002 abolished FIP-gts, induced increase in S phase, and inhibited IFN-γ secretion. Akt, a downstream effector of PI 3-kinase was phosphorylated and activated by FIP-gts. Results show that FIP-gts is a potent activator in PBMC and its effects are mediated via cytokine regulation of PI 3-kinase.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2008.01.005