In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser

The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disru...

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Bibliographic Details
Published in:Applied surface science 2008-03, Vol.254 (11), p.3370-3375
Main Authors: Cho, Sung-Hak, Chang, Won-Seok, Kim, Jae-Goo, Whang, Kyoung-Hyun, Choi, Kyeong-Sook, Sohn, Seong-Hyang
Format: Article
Language:English
Subjects:
Biological and medical applications
Biological and medical sciences
Cellular structure and processes
Exact sciences and technology
Femtosecond laser
Fluorescence
Fundamental and applied biological sciences. Psychology
General aspects
Instrumentation. Materials. Reagents. Research laboratory organization
Laser ablation
Laser internal processing
Laser-plasma interactions
Living cell
Molecular and cellular biology
Nucleus
Photo-bleaching
Photo-disruption
Physics
Physics of gases, plasmas and electric discharges
Physics of plasmas and electric discharges
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Summary:The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18 h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630 nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2007.11.030