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In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser
The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disru...
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| Published in: | Applied surface science 2008-03, Vol.254 (11), p.3370-3375 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c367t-d5372eac1e3990f997f073a98764908adc89604f91e88d0f43db983203e159353 |
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| cites | cdi_FETCH-LOGICAL-c367t-d5372eac1e3990f997f073a98764908adc89604f91e88d0f43db983203e159353 |
| container_end_page | 3375 |
| container_issue | 11 |
| container_start_page | 3370 |
| container_title | Applied surface science |
| container_volume | 254 |
| creator | Cho, Sung-Hak Chang, Won-Seok Kim, Jae-Goo Whang, Kyoung-Hyun Choi, Kyeong-Sook Sohn, Seong-Hyang |
| description | The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed
in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18
h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630
nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells. |
| doi_str_mv | 10.1016/j.apsusc.2007.11.030 |
| format | article |
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in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18
h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630
nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells.</description><identifier>ISSN: 0169-4332</identifier><identifier>EISSN: 1873-5584</identifier><identifier>DOI: 10.1016/j.apsusc.2007.11.030</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical applications ; Biological and medical sciences ; Cellular structure and processes ; Exact sciences and technology ; Femtosecond laser ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; General aspects ; Instrumentation. Materials. Reagents. Research laboratory organization ; Laser ablation ; Laser internal processing ; Laser-plasma interactions ; Living cell ; Molecular and cellular biology ; Nucleus ; Photo-bleaching ; Photo-disruption ; Physics ; Physics of gases, plasmas and electric discharges ; Physics of plasmas and electric discharges</subject><ispartof>Applied surface science, 2008-03, Vol.254 (11), p.3370-3375</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-d5372eac1e3990f997f073a98764908adc89604f91e88d0f43db983203e159353</citedby><cites>FETCH-LOGICAL-c367t-d5372eac1e3990f997f073a98764908adc89604f91e88d0f43db983203e159353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20175493$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Sung-Hak</creatorcontrib><creatorcontrib>Chang, Won-Seok</creatorcontrib><creatorcontrib>Kim, Jae-Goo</creatorcontrib><creatorcontrib>Whang, Kyoung-Hyun</creatorcontrib><creatorcontrib>Choi, Kyeong-Sook</creatorcontrib><creatorcontrib>Sohn, Seong-Hyang</creatorcontrib><title>In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser</title><title>Applied surface science</title><description>The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed
in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18
h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630
nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells.</description><subject>Biological and medical applications</subject><subject>Biological and medical sciences</subject><subject>Cellular structure and processes</subject><subject>Exact sciences and technology</subject><subject>Femtosecond laser</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Instrumentation. Materials. Reagents. Research laboratory organization</subject><subject>Laser ablation</subject><subject>Laser internal processing</subject><subject>Laser-plasma interactions</subject><subject>Living cell</subject><subject>Molecular and cellular biology</subject><subject>Nucleus</subject><subject>Photo-bleaching</subject><subject>Photo-disruption</subject><subject>Physics</subject><subject>Physics of gases, plasmas and electric discharges</subject><subject>Physics of plasmas and electric discharges</subject><issn>0169-4332</issn><issn>1873-5584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKv_wEMuets12dmvXAQpfhSKgug5pNlJm7JNarJb7L93l4pHTwMzz_vOzEvINWcpZ7y826RqF_uo04yxKuU8ZcBOyITXFSRFUeenZDJgIskBsnNyEeOGMZ4N0wnBuaPRdj31y4hhrzrrHfWG7ta-88myRaXX1q2odXTdb9UIu1WLtLX7sa2xbSl-a9thQ5cHqujr_J0a3HY-ovauoa0afC_JmVFtxKvfOiWfT48fs5dk8fY8nz0sEg1l1SVNAVU2bOQIQjAjRGVYBUrUVZkLVqtG16JkuREc67phJodmKWrIGCAvBBQwJbdH313wXz3GTm5tHG9UDn0fJWRFBiXAAOZHUAcfY0Ajd8FuVThIzuSYqdzIY6ZyzFRyLodMB9nNr7-KWrUmKKdt_NNmjFdFLkb7-yOHw7N7i0FGbdFpbGxA3cnG2_8X_QDkvI5o</recordid><startdate>20080330</startdate><enddate>20080330</enddate><creator>Cho, Sung-Hak</creator><creator>Chang, Won-Seok</creator><creator>Kim, Jae-Goo</creator><creator>Whang, Kyoung-Hyun</creator><creator>Choi, Kyeong-Sook</creator><creator>Sohn, Seong-Hyang</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20080330</creationdate><title>In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser</title><author>Cho, Sung-Hak ; Chang, Won-Seok ; Kim, Jae-Goo ; Whang, Kyoung-Hyun ; Choi, Kyeong-Sook ; Sohn, Seong-Hyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-d5372eac1e3990f997f073a98764908adc89604f91e88d0f43db983203e159353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical applications</topic><topic>Biological and medical sciences</topic><topic>Cellular structure and processes</topic><topic>Exact sciences and technology</topic><topic>Femtosecond laser</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Instrumentation. Materials. Reagents. Research laboratory organization</topic><topic>Laser ablation</topic><topic>Laser internal processing</topic><topic>Laser-plasma interactions</topic><topic>Living cell</topic><topic>Molecular and cellular biology</topic><topic>Nucleus</topic><topic>Photo-bleaching</topic><topic>Photo-disruption</topic><topic>Physics</topic><topic>Physics of gases, plasmas and electric discharges</topic><topic>Physics of plasmas and electric discharges</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Sung-Hak</creatorcontrib><creatorcontrib>Chang, Won-Seok</creatorcontrib><creatorcontrib>Kim, Jae-Goo</creatorcontrib><creatorcontrib>Whang, Kyoung-Hyun</creatorcontrib><creatorcontrib>Choi, Kyeong-Sook</creatorcontrib><creatorcontrib>Sohn, Seong-Hyang</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Applied surface science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Sung-Hak</au><au>Chang, Won-Seok</au><au>Kim, Jae-Goo</au><au>Whang, Kyoung-Hyun</au><au>Choi, Kyeong-Sook</au><au>Sohn, Seong-Hyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser</atitle><jtitle>Applied surface science</jtitle><date>2008-03-30</date><risdate>2008</risdate><volume>254</volume><issue>11</issue><spage>3370</spage><epage>3375</epage><pages>3370-3375</pages><issn>0169-4332</issn><eissn>1873-5584</eissn><abstract>The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed
in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18
h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630
nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.apsusc.2007.11.030</doi><tpages>6</tpages></addata></record> |
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| ispartof | Applied surface science, 2008-03, Vol.254 (11), p.3370-3375 |
| issn | 0169-4332 1873-5584 |
| language | eng |
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| source | Elsevier |
| subjects | Biological and medical applications Biological and medical sciences Cellular structure and processes Exact sciences and technology Femtosecond laser Fluorescence Fundamental and applied biological sciences. Psychology General aspects Instrumentation. Materials. Reagents. Research laboratory organization Laser ablation Laser internal processing Laser-plasma interactions Living cell Molecular and cellular biology Nucleus Photo-bleaching Photo-disruption Physics Physics of gases, plasmas and electric discharges Physics of plasmas and electric discharges |
| title | In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser |
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