Use of a thiophene-based conducting polymer in microbial biosensing

Immobilization of whole viable Pseudomonas fluorescens cells was achieved on a graphite electrode modified with a thiophene-based conducting polymer. Microbial electrodes were constructed by the entrapment of bacterial cells on conducting copolymer matrix using a dialysis membrane. The biosensor was...

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Bibliographic Details
Published in:Electrochimica acta 2008-05, Vol.53 (12), p.4104-4108
Main Authors: Odaci, Dilek, Kiralp Kayahan, Senem, Timur, Suna, Toppare, Levent
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Conducting polymer
Electrochemical biosensor
Electrochemical polymerization
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial electrode
Pseudomonas fluorescens
Various methods and equipments
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Summary:Immobilization of whole viable Pseudomonas fluorescens cells was achieved on a graphite electrode modified with a thiophene-based conducting polymer. Microbial electrodes were constructed by the entrapment of bacterial cells on conducting copolymer matrix using a dialysis membrane. The biosensor was characterized using glucose as the substrate. As well as analytical characterization, effects of electropolymerization time, pH and temperature on the sensor response were examined. Finally, operational stability was also tested.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2007.12.065