Study of photodynamic and sonodynamic effect on A549 cell line by AFM and measurement of ROS production

• Published in: Physica status solidi. A, Applications and materials science Applications and materials science, 2008-06, Vol.205 (6), p.1472-1477
• Main Authors: Tomankova, K., Kolarova, H., Bajgar, R.
• Format: Article
• Language: English
• Subjects: 68.37.Ps; 87.85.−J; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects; Molecular and cellular biology
• ISSN: 1862-6300; 0031-8965; 1862-6319
• DOI: 10.1002/pssa.200778119

Atomic Force Microscopy (AFM) has been used to image the morphology of developing tumour cells and their processes. It is frequently reported that prior fixation is required for reliable imaging of cells with lower adhesive properties. In our work we used a dry scanner in non‐contact mode to image cell line A549 (human lung cancer cells). Scanning of this object is limited by construction of AFM, therefore we replenished the apparatus with an inverse optical microscope. We imaged the cancer cells before and after photodynamic effect (PDE) and sonodynamic effect (SDE) of photosensitizer ClAlPcS2. PDE was induced by an efficient light emitting diode (LED) source with total light dose of 15 J cm–2. The treatment of the cells with the photosensitizer leads to the loss of surface rigidity and eventually to dramatic changes of the cell shape. Moreover, PDE increases reactive oxygen species (ROS) at higher concentration of the sensitizer, and ultrasound exposition enhances that ROS production. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)