Biodegradation of textile azo dyes by a facultative Staphylococcus arlettae strain VN-11 using a sequential microaerophilic/aerobic process

• Published in: International biodeterioration & biodegradation 2009-04, Vol.63 (3), p.280-288
• Main Authors: Elisangela, Franciscon, Andrea, Zille, Fabio, Dias Guimaro, de Menezes Cristiano, Ragagnin, Regina, Durrant Lucia, Artur, Cavaco-Paulo
• Format: Article
• Language: English
• Subjects: Activated sludge process; Aeration; Amines; Aromatic amines; Azo; Azo dyes; Biodegradation; Bioreactors; Bonding; Carbon; Daphnia magna; Degradation; Effluents; Metabolites; Oxidation; Reduction; Staphylococcus; Staphylococcus arlettae; Strain; Textile effluents; Textiles; Toxicity
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2008.10.003

A facultative Staphylococcus arlettae bacterium, isolated from an activated sludge process in a textile industry, was able to successfully decolourize four different azo dyes under microaerophilic conditions (decolourization percentage >97%). Further aeration of the decolourized effluent was performed to promote oxidation of the degradation products. The degradation products were characterized by FT-IR and UV–vis techniques and their toxicity with respect to Daphnia magna was measured. The amine concentrations as well as the total organic carbon (TOC) levels were monitored during the biodegradation process. The presence of aromatic amine in the microaerophilic stage and its absence in the aerobic stage indicated the presence of azoreductase activity and an oxidative biodegradation process, respectively. TOC reduction was ∼15% in the microaerophilic stage and ∼70% in the aerobic stage. The results provided evidence that, using a single Staphylococcus arlettae strain in the same bioreactor, the sequential microaerophilic/aerobic stages were able to form aromatic amines by reductive break-down of the azo bond and to oxidize them into non-toxic metabolites.