Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the determination of belotecan in the plasma, urine, and bile samples of rats. Belotecan was analyzed with HPLC using a C18 column with fluorescence detector. A mixture of acetonitrile-0.1 M potassium phosp...

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Bibliographic Details
Published in:Analytical letters 2009-01, Vol.42 (1), p.68-83
Main Authors: Jin, Qing-Ri, Kim, In-Wha, Choi, Min-Koo, Shim, Won-Sik, Tian, Guang-Yan, Lee, Soohyun, Lee, Jonghwa, Kim, Dae-Duk, Chung, Suk-Jae, Shim, Chang-Koo
Format: Article
Language:English
Subjects:
Analytical chemistry
Belotecan
bile
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
HPLC
Other chromatographic methods
plasma
rat
urine
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Summary:A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the determination of belotecan in the plasma, urine, and bile samples of rats. Belotecan was analyzed with HPLC using a C18 column with fluorescence detector. A mixture of acetonitrile-0.1 M potassium phosphate buffer at pH 2.4 (25:75, v/v) and 0.2% trifluoroacetic acid was used as the mobile phase. The lower limits of quantitation (LOQ) were 5 ng mL −1 for the plasma and 5 µg mL −1 for the urine and bile samples. The method has been readily applied for the routine pharmacokinetic study of belotecan in small laboratory animals.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710802568572