Immunological properties of Co gamma-rays irradiated bothropstoxin-I

We investigated the immunological behavior of BTHX-1, before and after irradiation. SDS-PAGE showed that BTHX-1 irradiated in the presence of NaNO3, had its structure preserved. Animals' plasma immunized with native BTHX-1 had high IgG1 titers. The irradiated protein induced high titers of IgG2...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2009-03, Vol.279 (3), p.817-821
Main Authors: Baptista, J A, Vieira, D P, Galisteo-Junior, A J, Caproni, P, Casare, M, de Andrade-Junior, H F, Spencer, P J, Nascimento, N
Format: Article
Language:English
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Summary:We investigated the immunological behavior of BTHX-1, before and after irradiation. SDS-PAGE showed that BTHX-1 irradiated in the presence of NaNO3, had its structure preserved. Animals' plasma immunized with native BTHX-1 had high IgG1 titers. The irradiated protein induced high titers of IgG2b. When the toxin was irradiated with t-butanol, there was a slight decrease in the production of IgG2b. Real-time PCR showed that both the IL-2 as for IL4 was more expression from the cells of the animals immunized with BTHX-1 irradiated. These results indicate that irradiation of proteins leads to significant structural modifications.
ISSN:0236-5731
DOI:10.1007/s10967-008-7416-z