Pulsed laser ablation of pepsin on an inorganic substrate

Pressed pepsin pellets used as targets were ablated with the pulses of the Nd-YAG laser. The activity of the pepsin thin layer, deposited on a glass substrate, was successfully detected by analyzing the proteolytic degradation areas on the polyacrylamide gel (PA-gel) copolymerized with albumin from...

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Bibliographic Details
Published in:Applied surface science 2009-03, Vol.255 (10), p.5458-5460
Main Authors: Cicco, N., Lopizzo, T., Marotta, V., Morone, A., Verrastro, M., Viggiano, V.
Format: Article
Language:English
Subjects:
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Laser
Material
Physics
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Summary:Pressed pepsin pellets used as targets were ablated with the pulses of the Nd-YAG laser. The activity of the pepsin thin layer, deposited on a glass substrate, was successfully detected by analyzing the proteolytic degradation areas on the polyacrylamide gel (PA-gel) copolymerized with albumin from the hen egg white (ovalbumin), used as an enzymatic substrate.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2008.08.069