Short communication: Keratinolytic potential of a novel Bacillus sp. P45 isolated from the Amazon basin fish Piaractus mesopotamicus

Bacillus sp. P45, isolated from the intestine of the Amazon basin fish Piaractus mesopotamicus, showed proteolytic activity when grown on skimmed milk and feather meal agar plates. The keratinolytic potential of this strain was evaluated on whole feather broth and human hair broth. Bacillus sp. P45...

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Bibliographic Details
Published in:International biodeterioration & biodegradation 2009-04, Vol.63 (3), p.358-363
Main Authors: Daroit, Daniel J, Correa, Ana Paula F, Brandelli, Adriano
Format: Article
Language:English
Subjects:
Agar
Amazon Basin
Bacillus
Bioconversion
Biomass
Broths
Degradation
Enzymes
Feathers
Fish
Hair
Hydrolysates
Keratins
Microorganisms
Milk
Piaractus mesopotamicus
River basins
Strain
Wastes
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Summary:Bacillus sp. P45, isolated from the intestine of the Amazon basin fish Piaractus mesopotamicus, showed proteolytic activity when grown on skimmed milk and feather meal agar plates. The keratinolytic potential of this strain was evaluated on whole feather broth and human hair broth. Bacillus sp. P45 degraded almost 90% of chicken feathers after 72 h of submerged cultivation on whole feather broth, and the production of extracellular proteases was observed. The formation of thiol groups was also detected during growth, indicating the contribution of sulphitolysis to the efficient hydrolysis of feather keratin. Nevertheless, Bacillus sp. P45 was unable to degrade hair keratin, possibly due to the conformational diversity of this substrate in comparison to feather keratin. Additionally, preliminary results demonstrated that this strain might be utilized in the degradation of recalcitrant collagen-containing wastes. The keratinolytic character of Bacillus sp. P45 might be utilized in environmental-friendly processes such as bioconversion of waste feathers, representing an alternative way of waste management that could lead to the production of value-added products such as microbial biomass, protein hydrolysates and proteolytic enzymes.
ISSN:0964-8305
DOI:10.1016/j.ibiod.2008.11.008