Synthetic diamond films as a platform material for label-free protein sensors

In the framework of developing a fast and label‐free immunosensor for C‐reactive protein (CRP) detection, H‐terminated nanocrystalline diamond (NCD) was functionalised with anti‐CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real...

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Bibliographic Details
Published in:Physica status solidi. A, Applications and materials science Applications and materials science, 2009-03, Vol.206 (3), p.520-526
Main Authors: Bijnens, Nathalie, Vermeeren, Veronique, Daenen, Michaël, Grieten, Lars, Haenen, Ken, Wenmackers, Sylvia, Williams, Oliver A., Ameloot, Marcel, vandeVen, Martin, Michiels, Luc, Wagner, Patrick
Format: Article
Language:English
Subjects:
68.08.−p
81.05.Uw
84.37.+q
87.85.fk
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Tissues, organs and organisms biophysics
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Summary:In the framework of developing a fast and label‐free immunosensor for C‐reactive protein (CRP) detection, H‐terminated nanocrystalline diamond (NCD) was functionalised with anti‐CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real‐time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC‐labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 μM CRP were recognised with a reaction time of 30 minutes. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1862-6300
0031-8965
1862-6319
DOI:10.1002/pssa.200880485