Vascular endothelial cells in cultures on nanocomposite silver/hydrocarbon plasma polymer films with antimicrobial activity

Bioactive hydrocarbon plasma polymer films containing 3, 30 and 39 at % of Ag in the form of nanoclusters were deposited on microscopic glass slides using an unbalanced magnetron with an Ag target operated in the dc mode in a working gas mixture of n-hexane and argon. The films were seeded with bovi...

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Bibliographic Details
Published in:Journal of Optoelectronics and Advanced Materials 2008-08, Vol.10 (8), p.2082-2087
Main Authors: Bacakova, L, Koshelyev, H, Noskova, L, Choukourov, A, Benada, O, Mackova, A, Lisa, V, Biederman, H
Format: Article
Language:English
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Summary:Bioactive hydrocarbon plasma polymer films containing 3, 30 and 39 at % of Ag in the form of nanoclusters were deposited on microscopic glass slides using an unbalanced magnetron with an Ag target operated in the dc mode in a working gas mixture of n-hexane and argon. The films were seeded with bovine pulmonary artery endothelial cells (line CPAE) or inoculated with bacterium Escherichia coll. The CPAE cells on films with 3 at.% Ag adhered and grew to a similar extent as on the control pure hydrocarbon films or glass slides. By contrast, the CPAE cell adhesion on films with 30 or 39 at. % of Ag was very low, and the cells died before day 7 of cultivation. In bacterial cultures, all silver-containing films attenuated the growth of E coil as opposed to pure hydrocarbon plasma polymer films.
ISSN:1454-4164