In Situ Bioconjugation: Single Step Approach to Tailored Nanoparticle‐Bioconjugates by Ultrashort Pulsed Laser Ablation

A single step approach to tailored nanoparticle‐bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides) is established. The integrity of oligonucleotides...

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Bibliographic Details
Published in:Advanced functional materials 2009-04, Vol.19 (8), p.1167-1172
Main Authors: Petersen, Svea, Barcikowski, Stephan
Format: Article
Language:English
Subjects:
bioconjugation
nanoparticles
pulsed laser ablation
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Summary:A single step approach to tailored nanoparticle‐bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides) is established. The integrity of oligonucleotides after conjugation and the stability of bioconjugates in physiological media are investigated. Their size is tailorable via process parameters. This rapid and universal method may provide biochemists with various nanoparticle‐bioconjugates for screening the often unpredictable structure–function relationship. A single step approach to tailored nanoparticle‐ bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides), is established. Their size is tailorable via process parameters. This rapid and universal method may provide biochemists with various nanoparticle‐bioconjugates for screening the often unpredictable structure–function relationship.
ISSN:1616-301X
1616-3028
DOI:10.1002/adfm.200801526