Superfine Saengshik Improves Liver Protecting Effect Compared with Fine Saengshik in an Animal Model

• Published in: Journal of food science 2009-03, Vol.74 (2), p.H59-H64
• Main Authors: Kim, D.H, Song, S.B, Kang, W.S, Jeong, Y.H, Yoon, Y.S, Lee, Y.M, Chang, B.S, Lee, K.J
• Format: Article
• Language: English
• Subjects: acute course; agglutination necrosis; Animal Feed; animal models; Animals; antioxidant activity; antioxidant effect; Antioxidants; Biological and medical sciences; Carbon Tetrachloride - toxicity; Comparative analysis; Diet, Macrobiotic; dietary supplements; Edible Grain - chemistry; Effects; Food industries; Food science; Food, Organic - analysis; Food, Organic - standards; Food, Organic - toxicity; functional foods; Fundamental and applied biological sciences. Psychology; glutathione peroxidase; Glutathione Peroxidase - metabolism; glutathione reductase (NADPH); Glutathione Reductase - metabolism; hepatocytes; hepatoprotective effect; hepatotoxicity; human diseases; human nutrition; inflammation; Liver; Liver - drug effects; Liver - enzymology; Liver - pathology; Liver - physiology; Male; Mice; Mice, Inbred ICR; necrosis; particle size; particle size distribution; Studies; superfine saengshik; Vegetables; Vegetables - chemistry
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/j.1750-3841.2008.01065.x

This study was performed to compare the effect of liver protection of fine saengshik (FS) and superfine saengshik (SS) and uncooked and powdered grains and vegetables, produced by the different mill technique on the acute hepatotoxicity induced by CCl₄ in mouse. As the result of particle size distribution in number, particles included under 0.955 μm dia were 7.02% and 68.92% respectively. Hematological and serological examination showed that AST (P < 0.05) and ALT (P < 0.05) of SS + CCl₄ group decreased significantly compared with those of FS + CCl₄ group. On the examination of antioxidant effect, water extract of SS showed a higher superoxide dismutase (SOD)-like activity on the condition of the HX/XOD system than that of FS (P < 0.001). Also, the glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.05) activities in liver showed a significant difference between FS + CCl₄ and SS + CCl₄ groups. On the histological observation of liver, SS + CCl₄ group showed a mild reversible hepatocytic change and infiltration of inflammatory cells around the central veins, whereas FS + CCl₄ group showed severe agglutination necrosis by CCl₄ toxicity. These results suggest that superfine saengshik significantly improves liver protection effect compared with fine saengshik; its major mechanism is supposed to be the improved antioxidant effect of saengshik by reduced size of particles.