Indirect Photometric and Fluorometric Detection in High-Performance Liquid Chromatography: A Tutorial Review

A review of indirect photometric and fluorometric detection in high-performance liquid chromatography (HPLC) is presented. A functional definition of indirect detection, somewhat broader and simpler than what was often used to characterize these techniques, was employed for this review. Emphasis was...

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Bibliographic Details
Published in:Critical reviews in analytical chemistry 2007-04, Vol.37 (2), p.107-126
Main Authors: Shen, Xiaoxuan, Tomellini, Sterling A.
Format: Article
Language:English
Subjects:
Analytical chemistry
Changes
Fluorescence
high-performance liquid chromatography
indirect detection
indirect fluorometric detection
indirect photometric detection
Ion chromatography
Methods
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Summary:A review of indirect photometric and fluorometric detection in high-performance liquid chromatography (HPLC) is presented. A functional definition of indirect detection, somewhat broader and simpler than what was often used to characterize these techniques, was employed for this review. Emphasis was placed on distinguishing different approaches to indirect photometric and fluorometric detection in HPLC based on the interactions which occur between the analytes and the detectable components either within the column or postcolumn.
ISSN:1040-8347
1547-6510
DOI:10.1080/10408340600976531