Electrotaxis of lung cancer cells in a multiple-electric-field chip

We report a microfluidic cell culture chip that was used for long-term electrotaxis study on a microscope. The cellular response under three different electric field strengths was studied in a single channel microfluidic chip. Electric field (EF) inside the microchamber was numerically simulated and...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-08, Vol.24 (12), p.3510-3516
Main Authors: Huang, Ching-Wen, Cheng, Ji-Yen, Yen, Meng-Hua, Young, Tai-Horng
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cancer metastasis
Cell Culture Techniques - instrumentation
Cell Line, Tumor
Cell Movement - radiation effects
Cell Separation - instrumentation
Electrochemistry - instrumentation
Electrotaxis
Fundamental and applied biological sciences. Psychology
Humans
Lung cancer cell
Lung Neoplasms - physiopathology
Metastasis–electrotaxis
Microfluidic
Microfluidic Analytical Techniques - instrumentation
Micromanipulation - instrumentation
Radiation Dosage
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Summary:We report a microfluidic cell culture chip that was used for long-term electrotaxis study on a microscope. The cellular response under three different electric field strengths was studied in a single channel microfluidic chip. Electric field (EF) inside the microchamber was numerically simulated and compared to the measured value. Lung cancer cell lines with high and weak metastasis potential, CL1–5 and CL1–0, respectively, were used to demonstrate the function of the multi-field chip (MFC). The two cell lines exhibited greatly different response under the applied EF of E = 74–375 mV/mm. CL1–5 cells migrated toward the anode while CL1–0 cells did not show obvious response. Under the applied EF, cell orientation was observed accompanying the cell migration. Judging from the different temporal responses of the orientation and the migration, it is proposed that the two EF-induced responses may involve different signaling pathways.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.05.001