Dietary Intervention with AHP, a Functional Formula Diet, Improves Both Serum and Hepatic Lipids Profile in Dyslipidemia Mice

• Published in: Journal of food science 2009-08, Vol.74 (6), p.H189-H195
• Main Authors: Luo, Yangchao, Chen, Gang, Li, Bo, Ji, Baoping, Xiao, Zhenlei, Yi, Guo, Tian, Fang
• Format: Article
• Language: English
• Subjects: Analysis of Variance; animal models; Animals; antioxidant activity; antioxidant status; Antioxidants - analysis; Auriculariaceae; Aurricularia aurricula; Basidiomycota - chemistry; Biological and medical sciences; blood lipids; Body Weight; Carbohydrates; Cholates - administration & dosage; cholesterol; Cholesterol - administration & dosage; Cholesterol - analysis; Cholesterol - blood; Complex Mixtures - chemistry; Complex Mixtures - therapeutic use; Crataegus - chemistry; Crataegus pinnatifida; Diet; dose response; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - therapeutic use; dyslipidemia; Dyslipidemias - chemically induced; Dyslipidemias - drug therapy; experimental diets; fatty liver; Fatty Liver - prevention & control; Feeding. Feeding behavior; Flowers & plants; Food industries; Food science; formula diet; functional formula diet; Fundamental and applied biological sciences. Psychology; human diseases; human nutrition; Hydroxymethylglutaryl CoA Reductases - metabolism; hydroxymethylglutaryl-CoA reductase; lesions (animal); lipemic effect; lipid content; Lipids - analysis; Lipids - blood; lipids profile; Liver; Liver - chemistry; Liver - metabolism; Liver - pathology; Low density lipoprotein; Male; Malondialdehyde - blood; Medicine, Traditional; Mice; nitric oxide; Nitric Oxide - blood; nutritional intervention; Organ Size; plant extracts; Plant Extracts - chemistry; Plant Extracts - therapeutic use; Pueraria; Pueraria radix; Random Allocation; Rodents; Superoxide Dismutase - blood; triacylglycerols; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/j.1750-3841.2009.01241.x

Aurricularia aurricula, hawthorn (Crataegus pinnatifida), and Pueraria radix are well known for both traditional food and folk medicine. Each of the above 3 plants possesses a distinct pathway contributing to treat dyslipidemia. To develop a health-promoting diet against dyslipidemia, the polysaccharides from A. aurricula, polyphenol from hawthorn, and P. radix were combined to postulate as a functional formula diet (AHP) in the present study and its pharmaceutical effects and underlying mechanisms were elucidated in vivo. The dyslipidemia model associated with fatty liver was induced by cholesterol-enriched diet (CED) for up to 12 wk in male ICR mice. Mice were randomly divided into 5 groups, that is, regular diet (RD), CED, Xuezhikang treatment (positive control group, PG), low and high (150 or 450 mg/kg/d) of AHP treatment groups. Compared with the CED group, AHP groups maintained lipid profiles through lowering serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C), inhibiting the accumulation of hepatic TC and triglyceride (TG). AHP could also improve both serum and hepatic biochemical activity profiles including antioxidant status, serum nitric oxide (NO), and hepatic 3-hydroxy-3-methylglutary CoA (HMG-CoA) reductase levels. Hepatic histopathological examinations showed markedly decreased fatty deposits in the liver of AHP-treated mice, illustrating the ability to reverse a condition of fatty liver. Our study indicated that this functional formula diet would be a potent alternative as a health-promoting diet, simultaneously targeting on the complexity and redundancy of dyslipidemia.