Correlation between blood pressure, cytokines and nitric oxide in conscious rabbits injected with Leiurus quinquestriatus quinquestriatus scorpion venom

• Published in: Toxicon (Oxford) 2009-09, Vol.54 (4), p.471-480
• Main Authors: Abdoon, Nuzha A., Fatani, Amal J.
• Format: Article
• Language: English
• Subjects: Animal poisons toxicology. Antivenoms; Animals; Biological and medical sciences; Blood Glucose; Blood Pressure - drug effects; Body Weight - drug effects; Consciousness; Cytokine; Cytokines - immunology; Inflammatory mediators; Interleukin-8 - blood; Leiurus quinquestriatus quinquestriatus; Leiurus quinquestriatus venom; Lung - drug effects; Lung - pathology; Male; Medical sciences; Nitric oxide; Nitric Oxide - blood; Organ Size; Rabbits; Scorpion Venoms - immunology; Scorpion Venoms - toxicity; Scorpions; Serum - chemistry; Time Factors; Toxicology; Tumor Necrosis Factor-alpha - blood
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2009.05.009

Activation of the inflammatory response with the release and activation of pro-inflammatory cytokines is among the factors thought to be important in the pathogenesis of many deleterious inflammatory effects seen in case of scorpion envenomation. The released inflammatory mediators interact in the body with a large number of proteins and receptors; this interaction determines the eventual inflammatory effect of the venom. Thus, in the present study an attempt was made to map the time course of scorpion envenomation and correlate the effects observed on the cardiovascular and respiratory systems with the changes that could take place in the levels of selected cytokines and nitric oxide during the course of experimental envenomation. New Zealand white male conscious rabbits were prepared for blood pressure recording. Arterial blood pressure was measured from the left central ear artery while a cannula was inserted into the right central ear artery and blood samples collected at different time interval after venom injection for biochemical and hematological analyses. In general, subcutaneous injection of Leiurus quinquestriatus quinquestriatus venom caused a significant ( P ± 0.05) triphasic effect on BP consisting of an initial transient reduction, followed by an increase that peaked 2 h after venom injection, and a gradual terminal hypotensive phase. The significantly high serum level of IL8, TNFα ( P < 0.001) and nitric oxide ( P < 0.0001) observed in the present study supports the evidence for the role of these potent vasodilators in the terminal hypotension that is usually observed in humans and animals after envenomation.